Science topic

Running - Science topic

An activity in which the body is propelled by moving the legs rapidly. Running is performed at a moderate to rapid pace and should be differentiated from JOGGING, which is performed at a much slower pace.
Questions related to Running
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I am looking for some guidance regarding when to get people back to running post ACL reconstruction, and what would be the best performance targets?
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Running does not require an intact ACL. Changing direction does. Most surgeons do tests like the single-leg hop, Biodex quad strength, quad circumference and other measures of quad and hamstring strength to determine if the athlete is ready. Each athlete and each sport is different. Some docs wait 4 months, most wait 6, some wait a whole year. I like my patients to wait 6 months and complete the PEP program or the 11plus program. I let my athletes run treadmill at 11 weeks after surgery if rehab is on track. Targets are full range of motion, good strength, no pain, and no other issues.
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I have run MD simulation of a protein at different temperatures. I want to monitor the stability of each individual secondary structure element. For this analysis I will have to make an index group for each secondary structure element, however for this study what would be more suitable RMSD or RMSF ?
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Neither. If you want to describe changes in secondary structure, use a program designed for that purpose, like DSSP, STRIDE, etc.
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I've been told by professional coaches that it is important to set an incline in order to mimic outside conditions, and that the distance would be inaccurate with a 0% incline.  I also saw articles that said that was a myth, and that the article used to encourage inclines only used 9 participants.  Edit: thanks to Pedro in the comments!  This question is in reference to Jones and Doust (1996).  How supported is this paper's conclusions by other empirical work?  What should I search to find more information?  I'm investigating the body of literature myself, but would love any shortcuts or tips.  Thank you!
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Dear Laura, The incline of 1% really mimics the (lack of) air resistance imposed by outdoor conditions. At least for speed between 10 to 18 km/h this compensation for distance is necessary (according to the study you mentioned).
Look, we compared the speeds obtained during an incremental treadmill and a incremental track tests, and we could observe the same peak speed. The same for speed at 4mM of lactate. Cheers
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Dear all now I am working on the interaction between heavy metal and natural organic matter. I want to use NICA-Donnan model to analysis and stimulate the interaction. However, as a immature in this field, I do not know which software can running this model, and how can I get these softwares. Hoping someone can help me
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Ask Dr. Meindert Keizer  Wageningen University and Research for the program ECOSAT and the isotherm fitting program of Dr Kinniburgh.
Alternative: Ask Gustafsson Stockholm for his computerprogram program 
KeizerM, van RiemsdijkWH. ECOSAT.Wageningen: Department of Environmental
Science, Subdepartment Soil Science and Plant Nutrition: Wageningen Agricultural
htm].
[242] Gustafsson JP. Visual MINTEQ ver. 3.0/3.1. (2000) KTH Royal Institute of Technology,
 Kinniburgh DG, Tang CK. FIT; Technical ReportWD/93/23. Keyworth, Great Britain:
British Geological Survey, Natural Environment Research Council.; 1993.
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Hello.
I've already run my data for HSD / tukey by using SPSS software. I've plotted the graphs for means separation effects/ homogeneity (document attached).
Is it possible if the graphs showed the letter abc, abcd, bcd (more than 3 letters) for the significantly different?   
Thank you in advance. 
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Hopefully Healthy Syafina
results graph shows that the data does not have symptoms of homogeneity, because the difference data is abcd, bcd and abc is not great. Data conclusions do not experience the effects of homogeneity. homogeneity occurs between a with cd
may be useful, my respect
Is anyone aware of literature on the relationship between recreational running and psychosocial wellbeing from a public health perspective?
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Hello all, I am currently undertaking research which aims to explore the relationship between recreational running and psychosocial wellbeing. I am particularly interested in this area in the contexts of community development, positive psychology, social care, social policy, and social work. I would appreciate any information or expertise on the subject. Kind Regards, Vivienne Li
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Hi Vivienne, I found the following which is perhaps more directly related to your work: http://www.apa.org/monitor/2011/12/exercise.aspx http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1470658/ However, I wonder if you have looked at the Football Fans In Training (FFIT) programme which some of my colleagues have been involved with over the years here in the UK. This has shown the impact a physical activity can have on obese football supporters. By linking with the clubs that they support there is a social element to the trial etc which may useful to your study. Anyway, whether their findings are any help or, and I suspect this will be the case, the references of the publications link to work by others that may help I thought it may be of interest to your work. http://www.ffit.org.uk/page0/index.html Best Wishes  Camilla
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I get quite sharp bands of my amplicon, when I loaded some of it to check the PCR product. But when I electrophoresed the samples all together to elute them, although I got my band but it appeared as a smear from the well. 
1. I electrophoresed at 70V. Should I increase or decrease the running voltage?
2. Is it because of higher amount of sample?
3. Or is it because of issues with gel polymerization and EtBr?
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The problem could arise from the proteins (i.e polymerase or other proteins such as endonucleases in case you do a colony pcr) in your sample. Pol can still attach to some of your DNA and if you have other protein contaminats, you could have lots of smears infact. I think when you load less simply you dilute the contaminants and get sharp band. But  with higher loading smears appear as the concentration of contaminants are higher in this case. So i would recommend to use 0.01 percent to 0.05 percent SDS in your loading dye or treat your sample with this low level of SDS. I hope this will help to get the problem  solved. 
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I do get serial correlation and cross-sectional dependence when I run the model using  EVIEWS 8. I would like to know if there is a way to overcome this. I appreciate your comments on this.
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Hie Nosheen. To deal with serial autocorrelation, hetroskedasticity and cross sectional dependence in panel data go for the Feasible Generalised Least Squares (FGLS) and the Panel Corrected Standard Error (PCSE). The former works well ifT>N, while the latter is feasible when N>T. In STATA use the xtgls syntax for FGLS and xtpcse for PCSE.
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The agreement between reaction-board measurements and kinematic estimation of adult male human whole body centre of mass location during running. There are different possibilities to obtain it, however, I would like to know what's the best method, or the method considered as "gold standard".
Thanks a lot.
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Dear Marcelo,
I agree with Dario. anyway, this is a paper compared between methods of Measurements of vertical displacement in running, it maybe help you.
Gullstrand, L., Halvorsen, K., Tinmark, F., Eriksson, M., & Nilsson, J. (2009). Measurements of vertical displacement in running, a methodological comparison. Gait and Posture, 30(1), 71–75. http://doi.org/10.1016/j.gaitpost.2009.03.001
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for Western blotting expeiment
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Dear Majan,
You should have a better idea than me, as you have a greater knowledge as to the contents of your sample than I do. However, the gel running stage achieves several things: 
It separates out the components of your sample depending upon their size. This tells you how big the components of your sample are. It may also inform you how homogeneous your sample is (e.g. other species, impurities, aggregates). Your Western may reveal lots of other bands in your sample. If you didn't run a gel then you would be oblivious to them. I can't say whether that is useful to you without more information. 
If you do not require the separation step then it is perfectly possible to remove it. In this case you perform what is called a Dot Blot: http://www.abcam.com/ps/pdf/protocols/dot%20blot%20protocol.pdf
I hope that is helpful to you.
Best wishes,
James
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Looking into the reasons behind the difference in net efficiency of walking and jogging/running at the same speeds. Whole tissue level to cellular if that's appropriate. Also considering other factors in cardio-respiratory area.
Thanks. 
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Hi Jack,
you may also give a look to this paper. In particular, in section 4.3 you can find a comparison between walking and running at the same speed, and it could be a valuable resource to carry on your research.
Best
Matteo
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Hi there - I'm running assays on freshly isolated rat cardiomyocytes and am running into some issues with incomplete lysing of the cells. Cells are happy and contracting at time of plating and after agonist addition, but these cells stay completely intact after the addition of the assay lysis buffer. Is this a common thing? Is there an additive that will assist in the cell lysis without affecting the assay performance? Any insight would be much appreciated!!
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You could try 1% SDS for lysis and dilute it 10 times in the assay
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Looking for mean values for the physical performance measurable in younger athletes' physical development from 12 to 19 years of age: Speed (forward running), jumping and strength.
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If you could specify the sport I am happy to point you in the direction of some of the youth literature.
In rugby union for example my own research (currently in review) and Josh Darrall-Jones have these splits recorded.
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I am using SmartPLS 3 for analysis. When I am running simple PLS the measurement model looks fine but when I run PLSc composite relaibility of interaction term falls to .23. Also, the loadings of indictors of interaction term decreases to mere .3 . There is no problem in CR and loadings of predictor and moderator are even in PLSc. The value of R2 is also increasing in PLSc.
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Hi,
the difference between PLSc and PLS is that PLSc consistently estimates models where the constructs are modeled as common factors while PLS is only consistent for models where all constructs are modeled as composites. For a difference between common factors and composites see Rigdon (2012) and Bollen & Bauldry (2011)
Kind regards
Rigdon, Edward E. "Rethinking partial least squares path modeling: in praise of simple methods." Long Range Planning 45.5 (2012): 341-358.
Bollen, Kenneth A., and Shawn Bauldry. "Three Cs in measurement models: causal indicators, composite indicators, and covariates." Psychological methods 16.3 (2011): 265.
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I need to study the expression of several genes. There are two options. To do qPCR of all wanted genes in several samples during one run or to do qPCR only one gene in all samples during one run. What is better?
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To be more efficiente in the lab I think is better to use one primer in all the samples just to prepare only one mix and distribute it to all the samples... Perhaps your question can be more precise?
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Hi everyone, I apologize for what may be a very silly question. I am having some very basic questions in the simulation of a simple 3-bus radial system. This system is shown in Fig. 1 below My question is why is the power at 'load bus 2' not equal to the value set in the three phase RLC load. My parameters for RLC load are Presistive = 10kW and Pind=1kW. But if I carry out simulation and use the PQ measuring function block, the value I calculate is 8877 kW and inductive power is 1621 kVar. I am using Phasor mode to run the simulation. Would appreciate any feedback.
Aditya
PS: I have attached the model I have used in the question, if you would like to take a look. Please set R,X = 1440.
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Not all method can be used in radial system, and you can try Fast decouple method, or intelligent method.
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I am solving a nonlinear optics problem. I have a solution set for a parametric sweep from 900nm to 1500nm with a step of 5[nm]. This solution is obtained at "epsilon= eps0"  for a material in my model.  Now i want to define "epsilon= eps0+normE" and run the parametric sweep for the same range at same interval. While running for the wavelength of 900nm, normE should be from the earlier simulation value at 900nm. similarly for 905 nm, normE should be from the earlier simulation value at 905nm..and so on till 1500nm. So how do i call this in my study node.
Thanks in advance for support.
 
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i think you have to use comsol with matlab there is some commands that may help you in your problem.
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I tried to use mean group and pooled mean group estimator by Pesaran and Shin (1999) by using Stata 13 and Eview 9, I found that the sign of short and long run coefficient are not consistent(ofter in opposite sign).
When I performed hausman test, it reported that asymptotic property of hausman test is not satisfied
Any body knows what are suitable estimators available to estimate panel ARDL(p,q) with N = 6 & T = 48?
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Hi, did you try the Common Correlated Coefficients Mean Group? It is available on Stata witht he command xtmg and extends the Pesaran and Shin estimator by accoutning for cross section dependence and non unit roots. The only complication is that if you want to build an ECM then you have to do it by hand (with IV and time dummies could be a good solution).
I hope this was useful,
Piero
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I'm running a rtpcr to obtain the standard curve, however, I got 2 bands on gel electrophoresis  for the NTC and the samples. I repeat the test 3 times, I got the same result in the first and third test repetition, whereas the second test showed only double bands for 3 out of 4 samples and the NTC and the lowest concentration were clear (no band). What this could be?
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Hey,
Have you checked if your RNA is contaminated? you can run the RNA only on teh agarose gel to see if it is only RNA? 
Secondly, you should also go for DNase treatment of your RNA before continuing to RTPCR
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How can I test for endogeneity when I'm running fixed effects model in Stata?
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Not an answer to the question as asked but you may want to look at this previous discussion
and
This paper of which I am a coauthor argues for the flexibility of random effects
there is quite a lot there about endogeneity; we argue that a Hausman test does not distinguish between the appropriateness of fixed and random effects models ( we show how to get exactly the same results from either)  but indicates that the cross-sectional effect is different from the longitudinal and that you can do so much more with the random effects approach :
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I have cDNA samples with concentrations range from 99- 6700 ng/ul and 260/280 ratio of 1.5- 2.02. I run a rtpcr using some samples to conduct the standard curve, however, the -ve (blank) curve was always higher than the samples. Is this has to do with the concentration and purity ratio?
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Dear Wajd, 
I think this is of use to you: 
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why is there patellar femoral pain after running?
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Runner's knee can result from:
Overuse. Repeated bending or high stress exercises such as lunges and plyometrics can irritate the kneecap joint (patellofemoral joint). Overstretched tendons (tendons are the tissues that connect muscles to bones) may also cause the pain of runner's knee.
Direct trauma to the knee, like a fall or blow.
Malalignment. If any of the bones are slightly out of their correct position -- or misaligned -- physical stress won't be evenly distributed through your body. Certain parts of your body may then be subjected to higher stresses. This can cause pain and injury to the joints. Sometimes, the kneecap itself is slightly out of position.
Problems with the feet. Runner's knee can result from hypermobile feet (a condition in which the joints associated with the feet can be move more than what's normal), fallen arches, or overpronation (flat feet). These conditions in which the impact of a step causes the arches of your foot to collapse, may excessively stress joints and tissues of the knee, .
Weak thigh muscles or muscle imbalance. Weakness in thigh muscles causes a disproportional load on isolated sections of the knee cap leading to abnormal wear patterns and pain.
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Hi all,
We have several metagenomic datasets and we are interested in creating fragment recruitement plots. that show the alignment of the reads along the genome and the sequence similarity of the reads compared to the aligned region. For more info see figure 2 in the Plos publication here: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0050077
We have already mapped all the metagenomic datasets with bwa against the reference genomes, so running another blast run is a waste of time in my opinion. But the IGV viewer only shows the alignment of the read, but does not show the similarity score for all reads at once.
Any suggestions on how we can tackle this issue?
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I think you can plot the MAPping Quality with reads lengths from your sam file to get the same result.
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Mice (Balb/c) performing regular aerobic exercise training (voluntary wheel running during 18 days): when will PGC-1a (mRNA and protein) be upregulated and possibly be downregulated again, in terms of hours and days?
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If you want to study chronic effect you need to make a washout period after running wheel training (48-72 hours). If you want to investigate acute effects you can sacrifice animals around 1-2 hours after a single bout exercise session (for mRNA, for protein you need more time). The upregulation is not so high (2,3x) and for these reason you need at least 6-8 animals per group to see the differences. I am using the same primers used in these paper and works very well to analyze PGC1a mRNA in mice, http://www.ncbi.nlm.nih.gov/pubmed/22237023
Does anyone have any resources regarding the biomechanics of curve sprinting, right versus left leg?
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I am looking for 1) a good explanation of the forces on the inside and outside leg and 2) elite athlete training processes for foot placement and 3) muscle involvement.  I want to relate this specifically to training and component selection for a right versus left transtibial elite curve sprinter. 
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I would interject the need to train in both direction on the curve so as not to develop an inclined posture, especially if the runner is also running without curves. Dennis
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I need help in running geNORM on qPCR results for 10 housekeeping genes (HKGs). I did run qPCR on 20 different samples (RNA) with 1:100 dilutions. I am not sure whether I need to run qPCR with different dilutions (at least 5) or 1:100 would be enough to run geNORM for analysis of the data for HKGs? Before selecting 1:100 dilution, I run qPCR for 10 HKGs on one RNA template with 6 different dilutions (ten times) and selected 1:100 on the basis of PCR efficiency and R2.
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Dear Sami,
I guess that means you have a qBase+ licence in which case you can get support here.
There's a manual with info on the reference gene validation on page 46 but if that is not clear enough, I've found that the Biogazelle Tech support is pretty good.
Unfortunately, my licence expired so I can't lead you through it step by step.
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Hello,
I am really new in this area. When I import  N4BiasFieldCorrection from nipype.interfaces.ants, it works well. But when I run the python code, the problem shows : OSError: command 'N4BiasFieldCorrection' could not be found on host chenruideMacBook-Pro.local Interface N4BiasFieldCorrection failed to run.  Anything else I should import or download ?
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No competence
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I am interested on how to run a simulation on this CASE tool. Any other advanced information on how this application is used in the academy or industry is also welcome.
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Hello Fernando,
There is a more detailed explanaiition in the link below:
For me was useful.
BR, Igor
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i dont want the code i want it's logic. Thanks in advanced
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 Thanks A lot David
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I am running Analytical Size exclusion using a Superdex 200 Increase 10/300 GL column of protein samples at ~40uM concentration. The protein is known to form hexamers and dodecamers as well as other oligomers, with a monomer being ~70kDa.
I see a rounded hump in the mutant and the overlapping of peaks in the WT.
Do you have any idea what is causing this? and why the proteins are eluting at a different volume than expected?
Many thanks,
Ruth
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Do you see single band in SDS-PAGE? NO then run native. 
Alternatively run standards with the same protocol and make rough estimation of size of co-eluting peak.
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I am trying to fixed up error during pushover run in masonry infill model.after initial run, hinges formed in strut but suddenly it stop the analysis.I am not able to understand. Pls sombody help me.Your effort will highly appreciate. I have attached 2 model herewith. Pls give me solution. 
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Most probably that you defined either the stress strain curve for the diagonal strut wrong or you defined the plastic hinge (axial only) wrong. Also beware that all signs for stress strain or plastic hinges definition must be negative to represent only compression capacity for struts.
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I am trying to run a piecewise regression model, but I am not sure about the assumption to be accomplished before running the model. Do I need to evaluate linearity, normality and ...?  
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Dear Waed,
Not very much to complicate a task, the linear is needed. Normality of noise is needed for verification of hypotheses about the points of switching. If the points of switching are known, the task of evaluation decides simply enough. In this case estimations of parameters will be optimum at standard assumptions of regression analysis. If the points of switching are unknown, a task becomes difficult. In this case it is necessary to know regression is continuous in points switching or not. The method of decision depends on an answer for this question.
Best regards,
Arnold Korkhin
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I was running materials studio and using Castep for delithiated crystal I found energy for Li.33CoO2 to be same as CoO2 which I did by changing the occupancy in the cif file of Li.33CoO2 .Whats wrong here
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Gjf-files can be treated by Gabedit (http://gabedit.sourceforge.net/).
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I am trying to find a trend of temperature in a catchment area. I have run homogeneity test on six of my stations and all of these are inhomogeneous. Now what should I do?
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thank you sir
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I try to run a MP2 calculation and I end up with the following,
Error ENOSPC: The number of shared memory identifiers for the node has been reached.
I ran exetyp=check and I used those values compatible with 8 cores, but it does not work even though I increase those values.
Please help to resolve it. I attached the input file for the convenience.
Thank You,
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Thank you for your reply, but depending on the exetyp=check run prior to the optimization run did not show that much of memory. I am using 16 GB memory installed linux based computer system.
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Does anyone encourage problems running DiveRsity/wrireBoot function in R???
I used it successfully several months ago, and now it constantly report error:
> writeBoot(infile = data_genepop, outfile = "23032017_10_boot", gp = 3, bootstraps = 10, parallel = F)
Error in data1[data1 == 0] <- NA :
(list) object cannot be coerced to type 'double'
My test data is attached.
Thanks in advance for your suggestions!!!
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Ok. So as I wrtote earlier, after using readGenepop you will obtain variable of class 'list' which is unsuitable for writeBoot. Instead you need to import your data from your_file.gen to R. There are two problem with it:
1. your file have structure which is a bit problematic to import it straightforward to RStudio (or R generaly) and obtain the correct structure of data needed by writeBoot function.
2. try to use:
> data(Test_data, package = "diveRsity")
you will make new variable Test_data from sample data attached to diveRsity package.
Than using:
>View(Test_data)
You will see the structure of data which works with with writeBoot fuction correctly. I have found that the difference between your_file.gen structure and Test_data is number of digits in cells. Each of population cell is represented by three digits while in correct Test_data you will find that there are 6 digits in each cell. I manually changed each cell with random 6 digits and writeBoot function worked (ok. it did not work when i added three zeros in front of each cell e.g. '245' -> '000245', however i was dealing with other type of file, so maybe when you do it in .gen it will work). So what you need to do is:
A) correct cells to have 6 digits (if you want tu use argument gp = 3, otherwise if you use argument gp = 2, they should have 4 digits) in your_file.gen
B) upload your file like this:
gendata <- readGenepop(infile = your_file.gen, gp = 3, bootstrap = F)
C) now variable gendata is unusable by writeBoot function, so you need to address the proper element within this variable. You do it by adding $raw_data after variable name:
writeBoot(infile = gendata$raw_data, outfile = 'gen_output', gp = 3, bootstraps = 10, parallel = T)
In effect R will make new catalogue with result files in in. In case you have problems where R puts catalogues just search for 'gen_output' via search engine of your system.
I hope I helped you (It took some time to make it work, but eventually I made it work on my computer)
All the best
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How can the laser beam from one lens of LIDAR be projected on the same position of rail when the train is running?
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Beam steering is needed most likely.  
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Hi,
I perform Kmeans algorithm with k=3, at every run I get a new order for the centroid values:
a. mu = 79.41    151.34 185.45
b. mu = 185.45  79.41   151.34
c. mu = 185.45  151.34  79.41
so how I can stabilize the centroid order to get the correspond class label?
thank you
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k-means clustering algorithm
k-means is  one of  the simplest unsupervised  learning  algorithms  that  solve  the well  known clustering problem. The procedure follows a simple and  easy  way  to classify a given data set  through a certain number of  clusters (assume k clusters) fixed apriori. The  main  idea  is to define k centers, one for each cluster. These centers  should  be placed in a cunning  way  because of  different  location  causes different  result. So, the better  choice  is  to place them  as  much as possible  far away from each other. The  next  step is to take each point belonging  to a  given data set and associate it to the nearest center. When no point  is  pending,  the first step is completed and an early group age  is done. At this point we need to re-calculate k new centroids as barycenter of  the clusters resulting from the previous step. After we have these k new centroids, a new binding has to be done  between  the same data set points  and  the nearest new center. A loop has been generated. As a result of  this loop we  may  notice that the k centers change their location step by step until no more changes  are done or  in  other words centers do not move any more. Finally, this  algorithm  aims at  minimizing  an objective function know as squared error function given by: 
                                                                       
where,
                           ‘||xi - vj||’ is the Euclidean distance between xi and vj.
                           ‘ci’ is the number of data points in ith cluster.
                           ‘c’ is the number of cluster centers.
Algorithmic steps for k-means clustering
Let  X = {x1,x2,x3,……..,xn} be the set of data points and V = {v1,v2,…….,vc} be the set of centers.
1) Randomly select ‘c’ cluster centers.
2) Calculate the distance between each data point and cluster centers.
3) Assign the data point to the cluster center whose distance from the cluster center is minimum of all the cluster centers..
4) Recalculate the new cluster center using: 
where, ‘ci’ represents the number of data points in ith cluster.
5) Recalculate the distance between each data point and new obtained cluster centers.
6) If no data point was reassigned then stop, otherwise repeat from step 3).
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I have soundscape recordings from two different treatment groups and want to compare the sound diversity in 8-10 different frequency bands between groups. I have tried and cannot get the R based program published by Villanueva-Rivera et al. to run for me, so I am looking for an alternative method to accomplish the same outcome, preferably in Raven or a free platform. 
Thank you,
Adrienne 
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Hi Adrienne,
We used the Raven program in our 2010 paper (reference below or ask me for it if you can't get it). The program was easy to use and allowed for comparing the sound power in individual frequency bands. I think, though, that it cost $100 for one year of use, but that may have changed...
(Manley, G.A., Kraus, J.E.M. (2010) Exceptional high-frequency hearing and matched vocalizations in Australian pygopod geckos. J. Exp. Biol. 213, 1876-1885.)
Good luck
Geoff Manley
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Hello all!
I've been doing WB for a while and have obtained great results for >15 kDa proteins. Now I'm searching for a 13kDa protein, which I do find, but bands are strange and linked one to another (pic).  In same membrane GAPDH (37kDa) is perfect.
I was using pre-cast gels 4-20% but since results were odd I decided to cast them myself: I've done a tris-glicine 12,5% to get a greater separation of smaller proteins using 40% acryl/bis 29:1. Separating and stacking buffers were as usual but i've made new ones for this issue (I use distilled water and no milliq). I'm running also as usual 80V (stack) and then 120V, but I've also tried to run at different voltages like 80V the whole time. Results are always the same for this particular range.
 Another issue I've considered is the samples concentration that are different. I'm loading 15ug/well (I've started with 30ug) prepared with laemmli 4x and volumes range from 8 to 20ul. I don't know if this can be a problem, but like I said proteins > 15kDa are ok.  
Any help is appreciated! 
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Hi Patrizia.
These bands are looking really strange.
I also do WB and analysis of small proteins (13 kDa).
I think it could be a problem due to your voltage you use. I think on the lower end of the gel there is much more force for the proteins to run through. Maybe the gel ran too far to the end?
I do my SDS using mA as constant. Maybe you can try this, too? I usually have nice bands with 20mA/gel. It took approx. 1h to reach the lower end.
In my experience its better to give it some time because if you push your proteins too hard through the gel the bands are often/usually more smeared and not so nicely defined.
I hope this may help you.
Best regards!
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Hello to all, As I'm working with MOA 16.04 tool, and when I run the task for detecting Drift, I'm getting the bellow shown output, I wanted to know which file or data they have used to detect the drift? Are there any possible ways to detect the drift with a different data set ( custom )? if yes then how?
I hope I'm clear from my side. Thank you.
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Dear  Ritika Jani ,
Look the link, may be useful.
Regards, Shafagat
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The concept exists from very ancient times across the globe but today we are running behind it like anything and doing everything for it For example: leaving our comfort, ease, luxury and lifestyle.
Kindly comment...
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For as long as resources are consumed and wastes are involved, then, there will be need for effective control whether in terms of the acquisition or in terms of the usage of the resource. Therefore, the issue of sustainability cannot be wished away in a hurry. The whole wide world has become a global village, hence the difficulty in framing ignorance of the challenges that are being or to be faced with by all especially from the perspective of the advancement in technology.which has brought about very serious need for more caution with respect to the way we deal with the ultimate outcome of the output of technological empowerment. This then brings about the issue of sustainability that we are going to contend with for a long time to come if the human race is to be more comfortable and live a quality life always hoped for by all. Hence, those in the behavioural and social sciences have a lot of work to do with a view to guaranteeing a better future for all no matter the race, gender, colour, age, location, religion, legal systems, etc.
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The 1.5 mile Cooper run uses 0.1656xbody mass (kg) where does the 0.1656 come from? How is it calculated and adapted in further studies?
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It's calibrated for subjects for which VO2 max is measured. 
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I am trying to convert raster (grid format) to ASCII format to run a Maxent model and my values are changing, is that normal?
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I would avoid writing floating point data to an ascii file. i would be inclined including to reduce the number of floating points by converting your data to integer and then export that (assuming you dont need all of that precision).
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I want to calculate the emission spectra of a organic molecule. I done the calculation, But I don't know how to get the emission spectrum from that?
Basis set I used : opt cis=(singlets,nstates=6,root=1)/6-311g(d,p) geom=connectivity scf gfinput gfprint 
I run the calculation based on below attached conversation from RG.
Please anyone help  me to solve this issue. Thanks in advance....... 
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All calculated data should be in the chk file. Try to use Gaussain View to open the chk file. If you did the calculation under Linux OS, you should use formchk to transfer chk file to fchk file, then open it via Gaussain View. If GV report error when you open fchk file, then use txt editor or notepad to change the word 'independent' to 'independant'. 
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Recently I have been working on gellan gel and I have taken data regarding conductivity, viscosity and their temperature variations. Now I want to interpret these data theoretically and run some simulation to confirm that.
Can any one suggest me any software or any programming scheme that will be helpful in this aspect?
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Molecular dynamics (MD) simulation should be a good option.
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I need to run WRF model to Simulate the Mandi, Himachal pradesh rainfall and for this I want to select the domain containing only Mandi district because later I have to use the wrfout file in ANN to train the data. 
Area of Mandi is 3,951 km², Is there any way of selecting domain for such small area?
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Hi Abhishek,
If I understood correctly, you are aiming to do the analysis over very small domain size. I beleive you can try nested domain approach. You can set-up coarser domain over larger region covering entire India (so that you can account into the influence of large scale flows over HP and Mandi).  The second nested domain you can set it up over Northern India and 3rd nested domain covering Mandi and nearby area. I think you can set 3rd nested domain grid size aprx. 4KM so that you can resolve all the convective processes.
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Dear All,
I am trying to use a set of dimensionless equations in OpenFOAM, as in the post :
and in
I have run a case with dimension forms without any problem.
Attached please find the new solver I want to use. Everytime I run the case with the new solver, there exists an error as below. I have copied the controlDict out to my working directory and changed the dimensionSet from 1 to 0, but the same error shows up. I make a similar change to the dimensionedType.C, the "dimensioned<Type>::dimensioned" to "dimensioned<Type>::dimless
I wonder if any one could give me some help.
Thank you in advance.
Best Regards,
Bill
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 Dear Mr. Wang,
Have you solved your problem with solver? If yes, could you share it? I am working on similar problem.
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How fast can a male adult (of about 30 years old) run or walk when moderately or severely drunk (alcohol impaired)? Any studies or scientific literature linking the BAC to running speed impairment?
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The problem is fall. When he falls?
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I recently started working in a lab that uses Vicon Nexus 1.8.5 and wants to hook their Bertec force plates (type 4060-NC) into Vicon. Does anyone have any tips, suggestions, or resources for this process? Previously the force plates were run through a program called Motion Monitor. Any help would be greatly appreciated!
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Hi Zach. You can add force plates etc. in the "system" panel within Nexus. When looking at the system panel (over on the left-hand side of the screen) there will be an option for "add devices" towards the bottom. In here you can select force plates and Nexus already has the different types of force plates for Bertec, AMTI, Kistler loaded in. All you have to do is select the appropriate version from the drop-down list and then input the specific data for each of the channels associated with the force plate as well as the dimensions (this information will be located in the manual that came with the force plate). So long as Nexus can see the analog signal associated with the force plates (which it will of your AD box is working), the installation is pretty straight forward (so much so that I was able to do ours at ESU). Good luck.
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Is it necessarily to reach the normal 0.9-1, 1mmol/L or higher, 3-5mmol/L? Does the actual lactate level of an athlete influence the actual physiological state?
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Ideally you would want to achieve PAP neurologically and increase blood flow  but minimize blood lactate accumulation. Even though raising BLA acutely is not that taxing any change in pH should be avoided until actual competition. 
How to store data in different files in C?
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Suppose i have 25 optimization problems. I run 1000 iterations for every problem. I want to store the result of 1000 iterations of each optimization problem in different file. How can i do that in C with file handling functions without manually declaring 25 file pointers?
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Dear Muhammad,  your code is wrong because it does not allocate memory for fp[i]. It will be OK if you declare "FILE *fp[25];" Dear Kavita, Since you do not want to use 25 file pointers, you can use only one: #include <stdio.h> ... FILE *pFile = NULL; char szName[16]; int i; for (i = 0; i < 25; i++) {    sprintf(szName, "file_%02d.txt", i + 1);    if ((pFile = fopen(szName, "a+")) == NULL)    {         //Some error processing         break;    }    // Your output, e.g., using fprintf(pFile, ...);    fclose(pFile); } You will have 25 files named "file_01.txt", "file_02.txt", etc.
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Hello,
Recently I start running ccsd(t) calculations in g09 but the output tells that a variables are required for run the job. I am gamess user it don't give this problem.
I try searching the problem, but I didn't find any useful.
Anyone can help me with this problem? What is supposed to do? How can I enter the variables? 
Thank you for your time,
Joaquim Mª Rius Bartra
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There are some software that are much more efficient to do coupled cluster calculations. A standard is MOLPRO, but I use Turbomole, and Orca is also very good. 
      Best regards,
              Sergio
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I run a CV for an organic compound and oxidation peaks were appeared in the negative current range.
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Dear Mr. Simbu,
As John Willey says, negative currents usually correspond to reduction peaks in the usual accepted convention for cathodic reactions (negative currents) and anodic reactions (positive currents). In some cases, US accepted convention for current sign is the opposite, that means negative currents correspond to anodic reactions (oxidation) and positive ones to cathodic ones (reduction). Just check if your equipment is using the US convention. Alternatively, a way to check such issue is using a known redox compound (for example ferri/ferro cyanide 1 mM in KNO3 0.1M) and see what is happening. That means, when doing cyclic voltammetry you should observe the reduction of ferri/ferro scanning the potential to more negative values and the oxidation of ferro/ferri in the reverse scan (going to more positive values). If the current you observe in the reduction process is negative, your equipment is working in the usual way, if not, then it is working in the US convention of current signs.
Otherwise, have you think about electrocatalytic reactions to take place in your system?. 
I hope this comment can help you. Good luck.
Cheers
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I have a dataset and I want to classify it. Is it necessary to run the 10-fold cross validation technique for more than once in order to generalize my accuracy?
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You should use 10-fold cross validation to determine the optimal complexity of the classifier. Not to determine the generalization properties (like accuracy). 
So yes, you should used some "10-fold cross test" on top of the 10-fold cross validation in order to determine the a generalization properties (like accuracy).
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We obtained M13 ssDNA from NEB and transformed suitable host bacterial strain followed by phage preparation from the same. Phage was then used for purification of ssDNA by PEG precipitation and phenol extraction (detailed protocol was followed as given in Molecular Cloning by Sambrook and Russell) and was used as a substrate for enzyme assay. There was no activity found. However, when M13 ssDNA supplied by NEB was used for the assay, enzyme activity was detected. When both DNA samples (isolated in lab and supplied by NEB) were run on the gel, one isolated in lab was found to run slower than that from NEB (image attached). Is there something wrong with the way I am isolating ssDNA even though M13 phage used for this was prepared from ssDNA supplied by NEB?
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ssDNA from phage is circular but can be broken in the extraction process and migration in agarose gel is slower.
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Hi all, I'm interested to find out the blood lactate and pH responses to 10km running, within elite athletes if possible. Does anybody know this or is there a study that has shown these values. 
Thanks for your help!
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Will do! :)
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I'm looking at the feasibility of using publicly available data downloaded from websites such as http://connect.garmin.com. http://strava.com for research. I've found one paper -  Haney Jr, T. A., & Mercer, J. A. (2011). A description of variability of pacing in marathon distance running - that accessed 300 marathon profiles, but can't find any others.  Anyone know of any others?
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Thanks Pablo.  Forgot about this one too which is where I see this heading to a certain extent - Hirsch, J. A., James, P., & Robinson, J. (2014). Using MapMyFitness to place physical activity into neighborhood context. Frontiers in Public Health. doi: 10.3389/fpubh.2014.00019
Can anyone help identify the problem with my 2 dimensional gel eletrophoresis?
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I did 2nd dimension GE (12% resolving gel) with around 100 mic.gram of protein loaded on the 17 cm IEF strip. After running the gel till the Bromophenol blue dye reached at the lower edge, I continued to run it for another 1 hr at 25 mA for better resolution.The pattern of spots that I got after silver staining the gel was quit hazy, with long horizontal streaks(perpendicular to the direction of running of the gel), most prominent being at the low molecular weight marker band positions. However, very few distinct spots were also visible. I was wondering what could be the problem? Did I run the gel for exceedingly longer duration or the molecular weight marker had diffused in the gel or my protein sample had degraded.....or something else. Kindly comment.
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Smearing across the gel like that simply indicates that your isoelectric focussing step (first dimension) was non-optimal. If your IEF was fine and your SDS-PAGE step was suspect, you'd see vertical streaking. Similarly with degradation between first and second dimensions (though then the streaking would be..clumpier, because proteins usually degrade into discrete fragments). Running the gel longer won't change any of this, it'll simply mean some of your lower mol wt proteins will run off the bottom of the gel. What kind of protein are you running, and how are you solubilising it for the IEF step? Isoelectric focussing can be very (very) tricky to get right, especially if you've got a lot of membrane protein in your sample. Also, are you sure you're running the IEF step long enough? Unlike the second dimension (which separates on size), IEF effectively runs "to completion": once proteins reach their isoelectric point they stop moving, so you don't need to worry too much about running them too long. Slow, steady separation overnight is not unusual for the strip method (at low enough power so you don't cook the proteins).
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What is the best method to analyze the patients satisfaction with scale of national level? Or other suggestions for better running the process.
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The essence of any satisfaction survey is contrasting expectations with satisfaction, be it on a national or international level. And because expectations with services may vary across countries/cultures, this is an essential part that should always be included.
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I use an ANFIS toolbox to predict a parameter with anfis and this error has appeared.
Warning: genfis1 has created a large rulebase in the FIS. MATLAB may run out of memory if this FIS is tuned using ANFIS.
How can I solve this problem?
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This error happens when number of FIS rules is greater than 250. You should use a smaller FIS (smaller Number of MFs) and use genfis2 instead of genfis1
GENFIS1 uses the grid partitioning and it generates rules by enumerating all possible combinations of membership functions of all inputs; this leads to an exponential explosion even when the number of inputs is moderately large.
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we calculated some Gaussian data and we have found a negative activation energy for our reaction , what is the meaning of it ? We repeated the Gaussian running for 2 times and again we have obtained the same result .
reactant has negative total charge and product is neutral but  with hydroxide anion.
Dear all, has any body references for  like this reaction? I need really those references.
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Dear Fateme,
First, can you tell us which energy you are considering ? is this electronic energy E ? E+ZPE ? Free energy ?
As was pointed out already, if you TS is lower in electronic energy than you reactant (or product), then conduct an IRC calculation because you surely will find some pre-reactive complex more stable than your TS.
Sometimes, the TS iss higher in electronic energy but becomes lower once you take zero point energy (or thermal corrections) into account. This might happen for shallow TS, and can be interpreted by the fact that when dynamics is included, then the TS 'disappears'. However, it is important to check that this is not an artefact of your functional. I did find similar behaviors in organic mechanisms in :
Chéron, N.; Jacquemin, D.; Fleurat-Lessard, P. A qualitative failure of B3LYP for textbook organic reactions Phys. Chem. Chem. Phys., 2012, 14, 7170-7175. doi:10.1039/C2CP40438A
Regards,
Paul.
How to increment the select line of a 16 to 1 MUX in run time (Verilog) without using delay signals?
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I supposed to implement a 16 to 1 multiplexer whose select lines should be increased automatically in run time. Since I've to calculate time taken by the MUX to output all sixteen inputs, It would be unfair if I use delay signals. Could anyone guide me in this regard?
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You can synthesize your design using any synthesis tools like RTL Compiler or Design Compiler with standard cell library, you will get timing details of all paths.
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Running on a treadmill at a confortable velocity is a quasi automatic motion without the need for much cognitive control, while running in a natural environment with obstacles surely requires greater attention and increased cognitive activity.
Will running, in a natural environment, increase beta and gamma frequencies observed?
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There is not a lot of work done using EEG while exercising. The reason is that the physical exercise can lead to lots of artifacts in the data. You should however look at work from Dr. Charles Hillman at the University of Illinois (http://kch.illinois.edu/research/labs/neurocognitive-kinesiology/). His group has done a number of excellent studies looking at how exercise modulates EEG/ERP signals.
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I see big number of products (including footwear) dedicated to people involved in many different sports and can't find the research on topics in my question.
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I'm looking for data about running kinematics of the upper limb. I would like to know the joints and trunk movements in running in healthy people, but i just find publication about unhealthy or disable people.
Thanks a lot
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Richard N. Hinrich's work is classic on this topic; superficial google scholar search revealed 2 articles in Journal of Applied Biomechanics. I would bet that the vast majority of articles in this topic area refer to these.
Upper Extremity Function in Running. I: Center of Mass and Propulsion Considerations
Upper Extremity Function in Running. 11: Angular Momentum Considerations
Regards
Sivan
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I want known, what can I do in order to calculate the sport zones training for my runners.
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Good question
How can I determine the speed and heart rate from a lactate\speed (running) graphic at the 2 mmol and 4 mmol threshold?
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I know I have to use the Dmax method (or at least it's one method) but how do I do it? With what resources?
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Maybe this can help. http://www.nuigalway.ie/maths/jn/LACTATE/html/lactate-e.html
How to calculate running speed at a specific lactate interval in an incremental treadmill test?
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I was given an incremental running test starting at 10 km/h and adding 1 km/h every 3 min. Also lactate is measured every 3 min. How can I calculate from this data the matching speed when the blood lactate is 3 mmol/l?
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Dear João, you should apply a linear interpolation using two speed and it's lactate values (anterior and posterior the specific value - 3mM). Using Excel you could use the "forecast" function to do this.
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The running literature contains several good references for prevalence and incidence rates of lower extremity running injuries, but I cannot find any statistics about the public health costs of diagnosing, treating, and rehabilitating these injuries. I am especially interested in costs of knee injuries and stress fractures of the tibia and metatarsals
Any help is greatly appreciated. Thank you!
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Dr. Khan: thank you for searching and for forwarding the link, but I am hoping to find specific statistics describing the direct and indirect costs of running injuries. Thanks for the help!
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for example :
the heel contact phase to the toe contact phase (the loading response phase) is ... percentage in the stance phase of running.What percentage?
the toe contact phase to the heel off phase (the midstance phase) is... percentage in the stance phase of running.What percentage?
the heel off phase to the toe off phase (push off phase) is ...percentage in the stance phase of running.What percentage?
Two foot switches are used to record the events running in my research.
please help me. thanks
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The percentage of the stance and swing phase will depend on the speed. As fast as it is, lower each stance sub-phase will be.
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I and my colleague, John Hockey, would be interested in hearing of other researchers using a phenomenological, sociological-phenomenological or ethnomethodological approach to investigating the lived experience of runners. Any references gratefully received.
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Fascinating topic! I don't know of any academic research myself, but perhaps there are insights to be had in the autobiographies of long distance runners. Slowing down the pace a bit, the best ethnomethodology of walking I read was the classic and controversial piece by Harvey Sacks. Meanwhile, the most interesting and convincing phenomenology of walking I ever read was Stephen Dedalus striding along the beach, in James Joyce's Ulysses. Good luck to you and John Hockey in the research.
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Does the phenol red interfere with LDS or DTT?
Can I run the protein samples diluted in medium or not?
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Phenol-red may be a minor issue compared with proteins in the fetal bovine serum when running SDS-PAGE. So you have to wash cells before lyzing them in desired buffer.
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When I run VEDA4, the calculation result shows "Test Alternative Coordinates
2 alternatives; not good results" , and output of VEDA4 has no vd or vdf file.
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According to the Lacour Equation, Cost of Running (C)= (VO2 - 0.083) x v-1. The 0.083 ml·s-1·kg-1 value (= 5 ml·min-1·kg-1) is the V=2 value corresponding to the intercept of the VO2/V-1relationship, established by Medbo et al. (1988).
If I have these values of VO2 (ml·min-1·kg-1): 65%=33,3538; 75%=36; 85%= 37,1385. 
These speeds (Km/min): 65%=0,151666667; 75%=0,175; 85%=0,1983.
These the C: 65%=186,9481315; 75%=180,5714286; 85%=162,0700958.
Is it normal the higher the intensity the lower the energy cost of running (C)?
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You need to look a the energy cost per minute and energy cost per meter. Energy cost per minute does not tell you about efficiency but if you divide by velocity you get Energy cost per meter. There will be an optimum speed which for walking is 80 m/min. You could view this with the car analogy of the amount of fuel per hour does tell you about efficiency but the amount of fuel per km does.
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Can we keep it in refrigeration for longer shelf life?
Using Milli-Q Water as a solvent A for running HPLC.
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Dear Duyi,
Milli-Q water is a deionized or ultrapure water, and steriled from all contaminants, but you can not keep the solvent prepared from Milli-Q water for a long time in the refrigerator, maybe you can keep it in the refrigerator for only 1 week, after that you can not trust that solvent, since some microorganism may grow up and it influences your analysis in HPLC system. I hope that I could help you, bye,
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Many studies have investigated different soccer small-sided games formats in order to find the effect of rule changes in tactical, technical, physical and physiological performances. In almost every protocol, warm-up activities are proposed, i.e. sprints, running, ball contacts and other. However, I couldn't find any reference to this choice. What is possible to speculate about the influence of the warm-up and the warm-up settings in soccer small-sided games?
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During the warm-up the players gradually works towards a peak. In last last phase they will prepare their muscles for maximum explosive football actions. Exercises to be considered here are a simple form of acc runs and sprints, whether or not in combination with a competition element. This increases the muscle tone (pre-loading), which will have a positive effect on players maximimum explosiveness. Of  course, this type of exercise must be performed in underload, so that the muscles do not become tired or dmged unnecessarily during the w-up. An intensive w-up (relatively high intensity, smaller volume and high tempo) will help players prepare their body for this peak of performance.
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Eg: step width, step length, stride length - all absolute and relative
Split into male and female
Split into age or running levels
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Attached papers. There are a lot of articles. If you need more, tell me! 
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I recently ran samples hoping to get microRNA on a 20% polyacrylamide gel, and I got some bands. The confusing part was that they came in at the highest band of a 5bp ladder, which was run with it. Can this still be microRNA?
Thanks in advance!
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I found very clean small RNA including miRNA bands on a 3-4% high resolution molecular biology grade agarose gel. You can also run total RNA as control to visualize ribosomal RNA bands on the same gel. Please check the materials and methods and also supplemental info on this paper. In case need more info, let me know. Thanks
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According to strain-stress diagram of the Achilles tendon, one can consider one linear and one nonlinear part before rupture. It is important to see which of these two area should be considered in vibration analysis. 
Is there any study (book, paper, etc) reporting that how much strain (what percent) occurs during running? 
Thank you in advance
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Hi Arash, the following may give you some idea altough its quite difficult to determine the strain using ultrasound during dynamic activities such as running
J Exp Biol. 2014 Sep 1;217(Pt 17):3159-68. doi: 10.1242/jeb.100826. Epub 2014 Jun 19.
Tendon elastic strain energy in the human ankle plantar-flexors and its role with increased running speed.
Lai A1, Schache AG1, Lin YC1, Pandy MG2.
Cheers
Steve
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I'm running 2D SDS-PAGE after immunoprecipitation. I'm eluting the protein complex with IPG strip rehydration buffer. However I feel I'm not able to complelely elute the complex from bead. Any suggestions on how to optimize the elution before I proceed for 2D? Thanks in advance.
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Hi Kamalika and Murat,
I do not recommend to boil the samples but the beads after elution (so you will have the elution with your proteins and the "naked beads", the latter should be tested) in order to check if there is still something stuck to the beads; Here, since you will not use the beads for any further experiment, the carbamylation will do no harm.
8M urea is quite high. I think, I would go with Murats WB suggestion.
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I'm doing my last school work presentation.The work is based on improving the techniques and  performances of lifeguards in Valencia.I need articles about sand training (Thanks Emmanuelle),lifeguard openwater trainings, rescue techniques with jetspit...
Thanks for your time.
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you could start with this review
What is the differences between symmetric and asymmetric optimization bound in PSO?
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Dear All, Is there any difference between symmetric and asymmetric optimization in PSO? For example, I can run the algorithm on [-50, 50] and [0, 100]. Could we claim that the performance of PSO affect by the symmetric and asymmetric bounds? Regards, Ehsan
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Symmetric or asymeetric depends on the objective function but not on its bounds. For instance, f(x) = x^3 +x ^2 + ...... will definately give different answer when u run on [-a, a] or [0, 2a].Because it may be that optimum be located outside the bounds. Also Prof. Fancois and Prof. Husseimann rightly pointed out the query....
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Since Organizations are doing both, which one will help the companies in the long run. What is your opinion about this?
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Ajai,
Marketing has become so large a discipline that the traditional fringes are always  been extended. We shall see more of this as research progresses.
A basic difference between social marketing and societal marketing exists in the purpose of the marketing effort. Marketers in social marketing aim mostly to transform habits and attitudes from a perceived negative one to a more positive one. To make people shift from eating junk food, which is harmful to health in the long run, marketers target offerings that are aimed at  change in those habits. In Ghana, social marketing aims at encouraging the use of condoms, for an example, to prevent STDs such as aids. Elsewhere it is aimed at discouraging sedentary life stlyes and junk food (Boyland et al. 2011)
The objective of social marketing may not necessarily be profit oriented, but a preemptive network against unnecessary and preventable morbidity, which affects the individual and society adversely. It is not uncommon to see social marketing efforts been central government and donor funded. Societal marketing aims at long term profitability.
Societal marketing may simply refer to the practice where a company attempts to be socially responsive and responsible in the provision of good and services that best suit the needs of its clientele ( Crane and Desmond, 2002). A poorly constructed building that collapses on the inhabitants, a fake drug that has little or no active ingredient etc are not capable of being credited with societal marketing credentials.
It can be said that societal marketing should be profitable and sustainable in the long run. This argument is buttressed by the fact that consumers are discerning in the world today, with many choices available for need satisfaction. The opposite must also apply. Who will continue to patronize shoddy goods?
References
Boyland, E.J., Harrold, J.A., Kirkham, T.C & Halford, J.C.G. (2011) ‘The extent of food advertising to children on UK television in 2008’, International Journal of Pediatric Obesity, 6, pp. 455–461
Crane, A. Desmond, J (2002), “Societal marketing and morality”, European Journal of Marketing, 36(5/6) 548-569
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I run a Markov switching models and now I want to run Log-Likelihood ratio test to test the non-switching hypothesis (one state model) against a two-state mode. How this test run in eviews 8
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We need to establish whether  your Markov chain is stationary with constant transition probabilities or non stationary. For the stationary case , simple approaches exist unlike the non stationary case.
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This is my code
thread_loop_c(t,d)
{
begin_c_loop(c,t)
{
temp=NV_MAG(C_T_G(c,t));
C_UDMI(c,t,0)= temp;
}
end_c_loop(c,t)
}
But when I do the same for velocity and pressure gradient it is running fine. It only gives segmentation violation error with temperature gradient. Not able to detect the issue. 
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Use DEFINE_ADJUST to fill the UDMI with initial values, i.e. C_UDMI(c,t,0)=0; subsequently, use your code. do not forget to hook the function 'adjust'. May be this will help!
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I'm attempting to run a logit model on longitudinal data and am getting the message that one of my variables predicts failure perfectly. A number of observations are then excluded from the model and I get no estimation for the aforementioned variable. When I run a crosstab of the predictor variable and the binary outcome, however, it does not appear that it should predict failure perfectly.
Can anyone tell me what's going on here?
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A bivariate crosstab will not show the problem.  The following FAQ from Stata Corporation may help:
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I am running serum biochemical tests on rats that have been exposed to a particular plant extract, I found out my direct bilirubin was higher than the total bilirubin in the control and extracts group. Has anyone had a similar experience? What can be responsible for this?
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Unconjugated bilirubin comes from red blood cell destruction and is transported to the liver to be conjugated and excreted into the bile. Unconjugated plus conjugated bilirubin usually consitute total bilirubin. I cannot explain your results.
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I am running an IRT analysis on an instrument in XCalibre, and the analysis reports substantially different means for the items than those calculated in Excel? Is there some weighting happening of which I am unaware?
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As Alan suggested, there is indeed likely an algorithmic reason that Xcalibre might be calculating differently.  I know of one situation for sure.  A few weeks ago I received a support email with the same question, and the issue was that they were running a 5-point polytomous calibration on a sample of only 36. (!!!!)  Xcalibre automatically combines response levels with N=0.  In that case, there was, for example, an item where no one responded as 1 or 2, only 3-4-5.  The 1 and 2 levels were dropped and it was treated as a 3-option item, and since numbering starts at 0 or 1 (depending on if doing PCM or RSM approach), it gets renumbered.  So the researcher anticipated an item mean of 4 or so and it was reported as 2 or so.
I'd encourage you to contact the support team about the issue.
(Disclosure: I am the author of Xcalibre.)
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I have whole brain lysates, and I would like to run a western blot and probe for Metallothionein (mW: 6KDa).  If anyone has success with this protein, please can you tell me what worked for you?  I have been having trouble with this because this protein is so small.  If anyone has some advice on transfer conditions, blocking conditions, etc., that would be great.  Any advice is greatly appreciated. Thank you. 
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You need to run gels fit to resolve low molecular weight proteins to start with. I would recommend NuPage system, probably the Novex4-12% in combination with MES buffer (resolving 2.3kDa-160kDa). Make sure not to run off the front. For  your blot you need increased methanol in the transfer buffer for optimal transfer of LMW proteins (15%-20%(v/v). 
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Who can share coding for simplest ARL for Shewhart control chart ? I am still confusing with the simulation and theoretical understanding. Thank you.
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I agree...but it's important to take into account the difference between ARL in control (ARLi) and ARL out of control (ARLo). On the one hand the ARLi should be as big as possible because you want the minimum number of false alarms (for a Shewhart type control chart ARLi=370). And on the other hand the ARLo should be as small as possible because you want to detect the process goes out of control as soon as possible.
If you are a beginner practitioner read this:
Montgomery, D. C. (2009). Introduction to Statistical Quality Control. John Wiley & Sons Incorporated.
And for the advanced ones:
Klein, M. (2000). Two alternatives to the Shewhart (X)over-bar control chart. Journal of Quality Technology, 32(4), 427–431.
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I know that support vector machine runs well in Matlab. But I have to know how well it works in java and which is easier to connect with NS2.
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MATLAB is the easiest way to implement SVM. Java is also a good idea but the only problem is that coding needs to be done and the number of lines of code in Java is more than MATLAB.
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Hello!
I want to look at telomerase activity levels on some cell line samples. I want the quantitative aspect of RT-PCR, but was wondering if I could also see the laddering effects of telomerase activity on a gel after I finish running the RT-PCR.
Particularly, I have been looking at the TRAPeze RT Telomerase Detection Kit. Can I verify telomerase activity on a gel with the RT-PCR sample products from this kit?
Thanks in advance!
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Okay, thank you very much for your advise! I am planning to run a positive and negative control in my TRAPeze RT Detection and then I wanted a gel image to further confirm the telomerase expression levels. 
Thanks so much for the help!
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Kinetics and kinematics variables 
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However a factor, remember one shining example of leg asymmetry: 8-time Olympic Champion Usain Bolt (!)
How to determine deep water running intensity training?
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Hello, does anyone know a field test to determine the training intensity of the deep water running/high knees exercise? 
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Polar claim that their HR monitors will work in freshwater, various anecdotal reports (blogs and forum discussions) indicate that it is not that reliable. If your aim is to maintain aerobic fitness during recovery from injury, RPE will achieve the desired effect. 
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Hi
already done the MD production so I want to analyse the helical
>>> structure of my protein by using do_dssp program.The command is like
>>> below :
>>>
>>> do_dssp -s md_18.tpr -f md_18.trr -n mainchain.ndx -sc
>>> dssp_scount.xvg -a dssp_area.xvg -ta dssp_totarea.xvg -aa
>>> dssp_averarea.xvg
>>>
>>> but after i run the command the result show like :
>>>
>>> Reading file md_18.tpr, VERSION 4.5.5 (double precision)
>>> Reading file md_18.tpr, VERSION 4.5.5 (double precision)
>>> Segmentation fault (core dumped)
>>>
>>>
>>> I already install the dssp program
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Thank you  for your guidance
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A 55kDa protein when run on SDS-PAGE in the presence or absence of reducing agent runs at monomer mass when boiled however it hardly enters the resolving gel when not boiled in presence or absence of DTT/B-me. what does it indicate?
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It means most probably that your protein aggregate after protein sample boiling. You should centrifuge the protein sample after boiling to get rid of the formed unsoluble aggregates ...
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I just synthesized HPMA monomer under the condition that Ulbrich et.al. did. In the first run, I get crystalline monomer but in the second run, I just get a sticky mass.I wonder if the monomers get polymerized?
the reference based on which I worked is:
Ulbrich K, Subr V, Strohalm J, Plocova D, Jelinkova M, Rihova B. (2000) Polymeric drugs based on conjugates of synthetic and natural macromolecules I. Synthesis and physicochemical characterisation. J. Controlled Release 64(1-3), 63-79.
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Hi Rajesh, 
thanks for your advice.Yes you are right, I follow the method mentioned in the reference...I'm going to repeat the reaction and see what gonna happen...
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Diffrent latitudes on Earth have different velocities relative to each other. This is because the velocity is less at other parallel latitudes than at the equator. Have experiments confirmed this observation (not null experiments), with real time clocks?
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Difference between what? You need to be more specific. If the planet was all water, clocks would run at the same speed everywhere on the surface even if the planet was rotating. The real planet has rock and water and both flow. You also need to be clear about whether you mean on the surface or at fixed radius from the centre. Bottom line is that the theory works perfectly and allows the dynamic maps produced by GRACE to show the actual gravitational field.