In my lab, hydroxyl radical scavenging activity of plant extract was checked based on Klein et al, 1992. but for us the absorbance value for samples increased more than the control.
My question is
1. why is the absorbance of the sample more? we used DMSO for sample preparation. is it because of that?
2. what has to be used as blank? we used Nash reagent as blank, is this right?
please clear my doubts..... it will be more useful for me.....