Science topic

Happiness - Science topic

Highly pleasant emotion characterized by outward manifestations of gratification; joy.
Questions related to Happiness
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Real standard comparison
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For an excellent survey measure of wellbeing, which takes account of both hedonic and eudaimonic perspectives, try using either the 14 item WEMWBS (Warwick Edinburgh Mental Wellbeing Scale), or the 7 item short version. It has been validated and is used as the Scottish national indicator for measuring population wellbeing. Many UK surveys include the measure, including Understanding Society, the largest social survey cohort.
The UK government has also identified four questions to assess subjective wellbeing, and these include a life satisfaction measure. A cautionary note RE satisfaction measures however: how people respond is influenced by what they expect and by their characteristics. To illustrate this: an English survey of cancer patients found that patients with prostate cancer were more satisfied with hospital food than patients with breast cancer. Of course the food they had was the same, the difference was that one group consisted of older men, and the other was female and somewhat younger. This is why health services research asks about specific experiences (e.g. were you offered a choice of meals?) rather than just how 'satisfied' they were.
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BIology researcher
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Being disease free is certainly a contributor to happiness but probably not the primary one.  Consider the difference between two communities - one consists of people who are out of work and living in poverty because their main employment source has been closed and even moving somewhere else takes money they don't have.
The other community is a cohesive community where people work together on common projects that support the community and where people have friends and neighbors that they can turn to for support if they need it.  Which community do you think has the most happy people?  Research data shows that health disparities are strongly associated with income inequality.  Social cohesion is positively associated with health.
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We are currently using a HemaVet 950FS which allows us to get accurate counts and differential from only 20ul of blood and are very happy with the consistency of the results we get. However, we seem to be having major issues with the reliability with which we get the reagent packs delivered. We would now like to explore alternative machines. Does anyone have any recommendations?
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Please take to Jeff Bailey to get a reliable reagent source.  We also switched the company.
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I'm looking to run an online version of a cognitive psychology study involving executive functioning tasks and a questionnaire.
I'm happy to code the tasks myself (part of the tasks are written for DMDX but I can also use another language or program), but am not sure about how to actually get them online so that I could just send participants a link to the tasks without them needing to download anything.
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Dear Emma, I used Inquisit for some of my tasks and had a good experience with the program and the service/help they provided. Hope this helps, Julia
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Greetings
I would be happy if someone could help me in mathematical modelling of the project.
Project details:
Stream: Food Biotechnology : Antimicrobial Peptide - Latarcin
Since we are nearing the deadlines for submission,I would be happy if I could get quick responses.
For more details, kindly mail.
Thank you
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Hello Dr.Gianluca Argentini
Thank you for the reply.
If you could provide me  your email ID, I would be happy to send you the papers and details relating to project at the earliest.
Shreenidhi
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Can any one explain me, the Homology 3D-structure protein PROVE plot what it will say and how we can calculate and which value is bad or better let me know the range? Please help me.I was stuck with this situation...
I modeled a structure of my interest protein. Next, for refinement and quality assessment, I submitted structure in UCLAsaves online server. Here it showed some parameters in that one of the parameters is PROVE plot. Can explain some one what range limits of this plot weather of our modeled structure good or bad.  
I would be happy if someone helps me.... 
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Please let me know if the following references/sites are useful to you:
1.  Structure determination of Leghemoglobin using Homology Modeling
by N Dhurigai - ‎2014 - ‎Related articles
Tertiary structures were determined by homology modeling software SWISS- MODEL an automated system for modeling the 3D structure of a protein from amino acid sequence using .... 184. Fig.V PROVE plot of Psophocarpus tetragonolobus ...
2.   Alternative approach to protein structure prediction based on ...
by Y He - ‎2015 - ‎Cited by 6 - ‎Related articles
Apr 21, 2015 - We demonstrate that ... tical 3D structures. ... homology modeling | amino acid physical properties | ... The prediction of protein structure from sequence is a central ... It can be seen from the plot that this approach gives 11.
3.  Homology Modeling of Nitrogenase Iron Protein of Nitrogen Fixing ...
by FB Sharon - ‎Cited by 2 - ‎Related articles
The nitrogenase iron protein 3D structure modeled by ModWeb was found to ... Nitrogenase iron protein, Homology modeling, Arthrobacter sp., Geno 3D, Mod ..... Ramachandran plot, minimized energy value, errat, verify 3D, Prove plot, prosa,.
Dennis
Dennis Mazur
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I would be happy if someone could give me some references to work (esp. Masterthesis) dealing with the problem of chemical milling of titanium. In particular for etching for wire fabrication. Thanks
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Thank you very much, you was a great HELP. I hope I could help you also one time. Best regards Walter
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I ll be happy to make a mathematical model for any type of statistical data. I am good at it. Please see my already published paper with Prof. M. Willander. Please let me know at saleem.khurram@gmail.com
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I am able to create 3D models as well. Very interesting
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Ever wonder where to start? how?  The CDC offers many challenges for subject matter experts to contribute to policy, monitoring, and other objectives.  Reporting illegal fishing is one way you can start to help conserve the globe. Happy to hear suggestions about other ways, too.
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The problem of conservation on a global scale transcends biology and any particular discipline. The solution seems incredibly simple: human laws must be subordinated to natural laws, ecosystem by ecosystem. But implementation is almost impossible because humans must stop being what we are. Global governance is conservative because every government representative is caught up in the consensus that put him in power. They prioritize the "me-here-now" instead of the necessary "all-everywhere-forever" (which few would vote for). The crisis is philosophical. The problem to be solved is governance.
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I am interested in the collaboration of Virtual Human Resource Development Research. Anybody wants help with their research.
   Happy to assit.
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Thank you
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I am wondering if there is any difference in Autobiographical Memory Retrieval Mechanisms in response to different age group? I would be very happy if anyone can suggest me any papers to answer that question.
Thanks.
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I hope that some of the articles in the attachment to the letter will be useful to you.
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I am stıdying on internal cycling of plant nutrients. I need some literaures on retranslocation- distribution, seasonal changes and kinetics of plant nutrients and biomass develop especially on Satsuma mandarin or citrus? I am also open for the other plants. I have many literatures but ı need newer (updated) ones. 
If you can help me , I will be so happy. 
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Please find enclosed some links and PDFs....
Seasonal Changes of Mineral Nutrients in Fruit and Leaves of ‘Newhall’ and ‘Skagg's Bonanza’ Navel Oranges, Article in Journal of Plant Nutrition 30(5):671-690 · May 2007
DOI: 10.1080/01904160701289347
Effects of leaf longevity and retranslocation efficiency on the retention time of nutrients in the leaf biomass of different woody species,Oecologia,April 1992, Volume 90, Issue 1, pp 80–87.
 
BORON UPTAKE AND DISTRIBUTION IN FIELDGROWN CITRUS TREE, Journal of Plant Nutrition , 34:839–849, 2011Copyright ,  onlineDOI: 0.1080/01904167.2011.544353
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Basically I'm trying to output the 3 MHz wave in air, but if there is a more reliable way to do that than a transducer, I'd be happy to know.
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Ultrasound generation to the air by traditional bulk piezoelectric transducers is limited by the impedance missmatch between the transducer and the air. This effect can be tuned out at a narrow frequency range by additional matching layers deposited on the active surface of the transducer. Another way improving largely the transducer to air matching is to use micromachined ultrasonic transducers (MUTs). There was done a lot of work on capacitive transducers (CMUTs) and on piezoelectric transcucers (PMUTs) that can work in the mentioned frequency range.
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I am basing a dissertation on this question. I am happy for any response, even if it seems a very obvious response. Thank you.
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I think, a social pedagogical approach benefits children in so many ways within an Early Years Provision. As we know, social pedagogy provides a holistic way of working with children and families in ways that support their well-being, learning and growth, for the most part. What is found centrally at the heart of social pedagogy is a belief that each person deserves to be cared for with dignity and possesses unique inner richness and potential, which we can help enrich them. Caring requires meaningful and reliable relationships that enable us to recognize a child’s potential, his/her qualities, strengths and interests, and to create learning situations in which children and families can experience their resourcefulness and develop new abilities to the best they can. That is why social pedagogy is often understood as instruction in the fullest sense of the term, as connecting the mind, heart, and hands. If we as professionals show such aspirations in our practice, we’re not only becoming better through our own endeavors; we also arrange a positive model for the children, an example that can encourage them to be more inspirational too.
Hope this helps,
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When we look at someone's photo (especially his/her face), we can have any feeling. Maybe we can feel happy, sad, angry, like, dislike, love, peaceful, joyful, fear, and other feeling/ mood. We will feel another mood when we look at the other persons photo.
What factors determine this circumtenses? Can this way be used as one of the media to treat mental illness? Depression, schizofrenia, bipolar, etc. 
When someone look at his/her favorit angel's photo or saint's photo maybe he/she can become more relax and tidy.
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The psychological effect of the eye view
Scientists have justified this phenomenon by looking at the eye for a long time, losing the connection to reality. Previous studies have shown the same effect when looking at anything until the dead, but this phenomenon was stronger when the focus of the eye in a human eye.
In terms of psychology, communication with the eye plays an important role in human relationships. The eye view is the foundation that builds trust between humans. In addition, avoiding eye sight when speaking, reveals lies. Looking at the eye helps to enhance self-confidence, and the confident look is usually admired by those around him, unlike the shy-looking person who can not look long in the eye of someone who talks to him.
The eye reveals happiness as it widens significantly and narrowed in anger and sadness. In cases of love, the pupil also widens and brightens.
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Setting up new lab, we will use the angle-two stereotactic robot from Leica. Most important: virus should be delivered at constant (slow) rate, low fluid dead volume preferred. 
I'm not happy with Hamilton-syringe based systems (needle too big), and I find it frustrating to load fluid into long tubes connected to microinjectors.
Any suggestions would be welcome!
Thanks, Johannes
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In our lab we use the Nanoject II, it uses a pulled glass pipet (diameter of 10um min) system
The virus is delivered in oscillator controlled pulses at a minimum volume of 4.6nl 
You do need to use a hamilton-syringe to load the glass pipet with oil but it's a very easy step, In our lab 5 people use this system without problem. I can send you more detailed information about the loading specs if you want.
There is a Nanoject III with touchscreen and other more modern features. But other labs have it and it has been causing problems, I prefer the more mechanical predecessor.
Hope it helps 
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happy to help with any contacts for PA roles as have some good contacts / experience regarding this non medical practitioner role. Let me know.
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Thanks Jill, I have completed my survey sadly at the time whilst a few replied the FPA body did not want to be involved and therefore declined to market survey to their members. However hopefully I will publish the results and further research will involve this group of professionals. Thanks for the offer. Jenny 
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Hi Martin;
Nice to connect each other. I'm glad that you are doing awesome jobs in food microbiology. If you can came to Chicago (October 1-4) at 18th Food Summit conference (http://food.global-summit.com/america/) for your speech, I will be very happy to see you. Byong (byong.lee@mail.mcgill.ca)
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I wish you good presentation
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I'm interested in such area of research and I would like to see the impact of social media on blood donation, especially in Saudi Arabia. and I'm more than happy to be part of the research team if you interested. 
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Yes I am planning to study this in the Middle East, especially in Turkey. I would like to be in touch for further studies in the Middle East. Thank you for your interest.
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Feeling so stress in doing research as it take up lots of time keep reading, writing and doing experiments, collecting data endlessly...
how do you cope?
thanks.
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Sometimes stress results when you think about the huge amount of work you must accomplish.  I suggest that you break down your project into small parts, and then only focus on the part you are working on NOW.
To use a metaphor, do not think about the tall mountain you need to climb.  Think only about getting to the top of the next hill.  
Of course, to do this you must have strong overall plan so you never pick the wrong hill.  :-)
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It seems that people who live in small cities take too much care about one another's life. On the other hand, those who live in big cities tend to express less interest in lives of their fellow citizens. Does it mean people who live in medium-size cities, on average, have the highest level of perceived happiness and life satisfaction?
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Dear Selman,
I know of no literature that addresses the topic you raise. See, for this respect, Dennis Mazur's answer. To be honest, I must confess that your considerations do not make much sense to me as a psychologist. As you say, it is more than natural that people who llive in small towns take more care of one another than those who live in medium-size and big cities. However, there is accumlated evidence that shows that to take care of others generally leads us to feel  happy and satisfied  Everyday life also shows that this is the case.Thus, instead of what you say --  "Compared to those from small and big cities, people who live in medium-size cities tend to be the happiest and most satisfied with their life", I would rather say that, compared to those from médium-size and big cities, people who live in samll towns tend to be the happiest and most satisfied with their life. Note that there is a lot of evidence that shows that if we need help, we get it more easily when bystanders are only a few, not many, This is called the psychological phenomenon of responsibility diffusion. In your considerations you speak about too much care about one another's life. As I see it, however much we are concerned with others' well-being we may say that such concern is never too a much concern.
It can be the case that you think that I and being a little rude.If this is the case, I didn't intend it. In orther words, instead of being politically correct, I prefered to be honest in intellectual terms.
I hole tis helps.
Best regards,
Orlando
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We would be happy to know more as we are provider for epigenetic services and looking for collaboration. We've been working already with C. Ospelt.
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Yes, I am doing this kind of research.
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I need to find giic for Al 2024-t3 or any other alloy of Al . gic is available on the net but i couldn't find giic or giiic.
I'll be very happy if anyone helps me with this.
actually i need them for solving my project in ABAQUS . I'm trying to use vcct method in an ENF specimen and i need critical strain energy release rates. 
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As a rule of thumb, normally G2c is 3 to 5 times larger than the G2c. It depends one the material but 3 to 5 times of G1c can be a rough estimation
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I have been researching vibroacoustics and it's use in healing chronic conditions for the last 5 years. Have met with indifference and scepticism from current health funding bodies in the UK so am developing the therapy with clients and learning from them. Have had some successes with clients and keen to collaborate with others
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Vibro-Acoustics, Volume 1(Springer)
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I tried Skeletonize in Fiji and Neurolucida 360, but was not 100% happy with both.
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Hi Danielle,
Skeletonizing alone is insufficient as you might see.
You could add other filters before doing that or rather use thresholded thinning instead of skeletonizing.
For instance a median filter before will level out intensity variance and then thinning and skeletonizing will work better.
If you do a thinning you can preserve the somata and get the real length of the processes (and the branching). However, I use Image Pro Premier for this (and don't have experience with Neurolucida). Next I use a limited adapted threshold for defining the threshold of the thinning algorithm (that in the end makes 1 pixel thick objects of all processes so that they can be counted).
And for classification to sub-types I do like Guy before suggested. I train the software using a set of different parameters (textural and common like standard deviation of signal) and then the system runs a factor analysis defining a recipe for cell type separation.
Hope this helps and is not too gobbledygook...
Best
Dan
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I am looking for Questionnaire for measuring happiness at work. Any previous study used happiness at work measurement?
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The following papers should be helpful to your topic:
  • Cropanzano, R. and Wright, T. A. (2001). When a "happy" worker is really a "productive" worker: A review and further refinement of the happy-productive worker thesis. Consulting Psychology Journal: Practice and Research, 53, 3, pp. 182-199.
  • Fisher, C. D. (2010). Happiness at work. International journal of management reviews, 12, 4, pp. 384-412.
  • Spicer, A. and Cederström, C. (2015). The Research We've Ignored About Happiness at Work. Harvard Business Review, available from: http://hbr.org/2015/07/the-research-weve-ignored-about-happiness-at-work.
  • Zelenski, J. M., Murphy, S. A. and Jenkins, D. A. (2008). The happy-productive worker thesis revisited. Journal of Happiness Studies, 9, 4, pp. 521-537.
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I have made a project about the measure "The Hapinnes" in Learning and Pedagogical strategies for improvement learning environment
If you know about that question please send me details and bibliography,
The purpose of the present research is to use Neurolinguistic Programming (NLP) using pedagogical strategies in virtual learning environments: (Games and Neurolinguist Assesment).
The use of the (NLP) as strategic-pedagogical contribution to improve the investigative competences in students in virtual learning environments has had very little use in the educational environment.
NLP has recently begun to be used to implement an education and learning model that strengthens the different pedagogical strategies in learning. With the new information and knowledge society and recent advances in ICT in education, the use of virtual learning environments has intensified in the incorporation and appropriation of innovative methodologies with NLP in virtual education. It seeks to incorporate virtual tools with the App with (NLP) that allow to promote learning autonomously on the part of the student.
Create a didactic strategy based on neurosciences that allow the student to be more conscious, motivate him to develop the autonomous capacity of virtual work to improve concentration, control himself, and obtain a higher academic performance in the learning of knowledge new. Its evaluation and measurement of the impact of the methodology and its statistical analysis was done through the statistical software SPSS
Apply the neurolinguistic programming strategies for cognitive development identified in neuroscience to incorporate them into the didactic devices in the improvement of the quality of the virtual teaching-learning process.
Thanks you very much for your helping.
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Happiness is that a person feels his existence with someone who loves or achieves a goal he has dreamed of achieving
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I will be happy if experimental alloys cheistry is known and expected mechanical properties and intended applications.
Regards
Shivraj Singh Kasana
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Hi, I would suggest to use Prof Ashby's books on Materials Selection for
some general answers and examples. For specific problems you should search the literature; see the answer you got from Dr L G Thomas.
GOOD LUCK.
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Up to what extent feeling of love and affection provide energy, activeness and happiness in human life? Is there any substitute of feeling of love and affection?
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One finding I recall from Martin Seligman's research is that they looked at which "character strengths and virtues" the happiest people tend to have, and they noticed that one of them was what they called "the capacity to love and be loved" - note that this is not quite the same with actually being in love or being loved, but still, a useful finding. For more about this, see the six references listed under my project "Critical geographies of health, happiness and well-being" at this link:
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Recent celebrations of All Saints Day in the christian world and Halloween in the secular world awakened in me serious thoughts of the Last things. At the end of life's journey, when all my strivings cease, and all of life's successes and achievements or failures recede in importance, what desire would occupy my heart? What is your deepest desire? I will be happy to know your views. Thank you.
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"Every actor's deepest desire is to reach a huge audience. So, I don't look down upon commercial cinema... there's a beauty in it that you understand sooner or later" - Randeep Hooda
"The deepest desire of the human spirit is to be acknowledged" - Stephen Covey
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Dear all,
Any of you has experience in doing bonding PDMS channels to a microelectronic chip? Could you suggest some papers about that?
I am basically interested in knowing which are the differences in bonding PDMS to a microelectronic chip instead of PDMS-PDMS or PDMS-glass bonding.
Additionally, I would be happy to have information about the bonding strenth in a PDMS-electronic chip bonding.
Please, note also that the chip that I am going to use as a substrate is 3.5mm x 3.5mm in size and has a passivation layer of polyimide on the top. It was fabricated using 0.35um CMOS technology.
Any suggestion is welcome,
Thank you!
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Hello Valerio,
Below are some resources you may find useful as a starting point.
Flexible packaging of solid-state integrated circuit chips with elastomeric microfluidics:
A multi-scale PDMS fabrication strategy to bridge the size mismatch between integrated circuits and microfluidics:
Lab-on-a-chip devices: How to close and plug the lab?:
Kind regards,
Jules
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I only find online the human dosage and i will be happy to find a good protocol to focus my calibrations...
thanks!
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Hi Narin,
For rifampicin our lab uses 20 mg/L. There are many agrobacterium mediated genetic transformation articles you can reference to. I am not sure if this is what you are looking for.
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Enjoyed briefing this delphi study report thnx. Were 67 potential BIM BoK line item sentence fragments further defined at some point before voting on?
If so I would be happy to review and comment on those.
FYI: I have benefited from crossing paths w/Deke Smith, Calvin Kam, Bilal Succar, and Brittany Giel all of whom are mentioned.
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Hello Matt, Thanks for your comment! We're currently in the process of developing an implementation guide of the proposed BOK, and I can share with you some working examples. We're planning on an update at our next annual conference, i.e. the 12th BIM Academic Symposium in Orlando (http://dcp.ufl.edu/cacim/conferences/bim-education/bim-education-2018/) to truly adopt the BOK for both educational and industry use cases. We're considering using the Developing a Curriculum (DACUM) instead of Delphi this time. We would love to have more subject matter experts like you to offer valuable insights.
Who can help in evaluation of quality of translation of some sentences (environmental science, ecology) from English into Chinese?
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I am lecturing in a Chinese university now. My students translate some sentences from English scientific articles. I would like to be more objective in evaluation of their performance. I would be happy to hear you opinion on the quality of their translation from English into Chinese. Thank you in advance. Key words: environmental science, ecology, Chinese,
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Qing Liang Meng , thank you very much. May we stand in touch? I am currently a Visiting Professor (Environmental Sciences) at a Chinese University. I do not speak Chinese. However I want to make more knowledge more available to Chinese students... Good luck to you.
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All Brains are created Equal ... was an ancient belief that our minds were all merely "blank slates" (tabula rasa). This one-minute brain scan clearly proves how remarkably different we truly are. Built on a bedrock of 40 years of research/testing experience, this brief "brain-scan" opens the door to honoring and communicating about individual thinking/feeling styles (your brain bias is as individualized as your unique facial features). Matching your mindset with others has a clear advantage (for example, the closer your mindset is to your professor's mindset, the higher your test scores & grades). Seeing eye to eye is key to happy relationships. We value those who think "Just-Like-Us." Knowing your mind’s “profile” will provide you with the chance "flex & stretch” your mind, like circuit training for your brain. You may have a creative side which has been underdeveloped because you had to think “inside the box.” Many “outside of the box” thinkers (those with learning differences, LD, gifted potential, highly creative,) may merely have a different “brain-operating system” which this brain-scan could detect. Even conventional testing today is embracing a broader, more whole brain approach (verbal & visual, convergent & divergent) as our understanding of our brain’s capacity is expanding. Understanding your baseline profile will also help you recognize when your brain is not hitting on all cylinders (caffeine, fatigue, hyperventilating and stress downgrade your brain's performance). Knowing yourself will help you optimize your performance by knowing when to rest and knowing when to engage. You will be more aware of when your brain has hit its best "stride", perhaps after exercising or meditating or just appreciating life.
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Hello Ron, 
I read your interesting abstract but still didn't think I knew much about it, but knew that you had stimulated my interest. So . . I looked for further info and carried out your left brain / right brain exercise, finding that I was left brain: 11 and right brain: 3. Your comments on the result are really interesting. 
I tried to open your 'One Minute Brain Scan REV3' (Oct 2017) on your ResearchGate page but couldn't open the pdf. Is it possible to send it to me? Thank you. I don't know if it's just me, or the RG link won't work.
Best wishes and thanks also for the stimulating read,
Mary
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Hi everyone! I wanted to know if anybody knows how we code random words as positive (+1), negative (-1) and neutral (0). Like how do we know if a word is positive, negative or neutral. For example, house, happy, hand, hall etc..Do we look at it's valence and if yes, how can we find the valence of a word? It is for a psychology research project. Many thanks :)
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Perhaps the easiest method is to have a sample of people rate the words from positive to negative, perhaps on a 7 point scale ranging from extremely positive to extremely negative with neutral in the middle. The mean response could be taken as the value. There are more complex ways to scale the words as explained in JP Guilford, 1954, Psychometric Methods.
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Very interesting goal, I will happy to contribute should our expertize on the topic will be needed. Feel free to ask whenever You want.
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The methodology we will use for this evaluation is a comparison to professional reference equipment available at the University of Stuttgart and a comparision to professional equipment used by the local authorities. The local authorities do measurements for legal reasons to ensure compliance with EU legislation with respect to the air pollution limits. However, we will also do a comparison between different brands of PM-sensors. What we are also looking for is the option to do a compensation for some of the inaccuracies that we will determine.
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We welcome MPH students in Sweden and outside Sweden using SAGE, SAGE-INDEPTH, SAGE-HIV WOPS, or any data sets to engage on this project - add yourself as a contributor, and contribute to the discussions here.  Use this as a portal to ask questions, raise issues with data and analyses, and engage with other data users.
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I have worked on a manuscript on mosquito net out door use (orthodox and unorthodox usage) to check mate vicious outdoor bites, foot ball goal post,plant fencing and shading.
A journal I sent it to wants me to send it to a professional who has extensive publication experience,there after I should re-send to the journal again.
I would be glad as an emerging scholar to learn from such experienced professionals. I will be happy to be guided to revise the manuscript if need be.Thank you
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Hi Kacey,
Thanks once more.Please find attached up loaded.Please feel free,looking forward to a critical review.
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Please answer these questions based on your own experience. Does one aspect contributes to the success of the other aspect/s? e.g. my family inspires me to become successful in my work. Or the other way around, e.g. I became successful in my work regardless of my family condition or I have a happy well provided family regardless of my work or friends. Thank you very much in advance for your responses.
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In my experience, I anticipate that each researcher will have his or her own motivations that push or drive them internally, ranging from purse self-motivation to motivations based on family and other significant others in their lives.  Some researchers will not even have families due to their dedication to their personal research effort.  Others researches will feel a need to prove themselves to his or her own family or significant other.  Some researchers despite the intense work, care, patience, required in research may exhaust their emotions on their research side depleting their emotional energy in their research field.  Some researchers may find research so difficult for them that they need their family support to keep going.  For others, research ideas and their execution may come easy and they have time to enjoy their lives with others.
Dennis
Dennis Mazur
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? above is mandated by Reaserchgate.)
I fondly remember my days in your lab many moons ago.
Winfried Raabe
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bye
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I have studied a producing mechanism of lupulin glands for more than 40 years. I am concentrate on this purpose from now. If I elucidated this mechanism, I would be so happy to get it. I am studying with this work by myself. It takes a long time to accomplish this work, I think. If somebody is interested with this work, I would like to study it together. 
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Dear Dr Takayuki Momma,
I am not working in this field.
Kind regards
Luitgardis
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In order to compare the adsorption of an organic compound on Zeolite, I am looking the kinetic diameter of 2-butoxyethanol. I couldn't find in literature. If someone knows where I can find this information, I would be very happy.
Thanks  
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Use software like MOPAC or ChemOffice to get the theoretical molecular dimension.
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Hi everyone, having a little bit of trouble here and I would really appreciate some help.
I am writing a study comparing the resistance profiles of bacterial isolates from patients presenting to a hospital, before and after implementing a new antibiotic stewardship policy in the hospital and surrounding practices.
My question is: even though the bacterial isolates taken before and after implementing the programme are independent (ie. not from the same individual, not the same bacteria), as I am assessing any differences that are due to implementing the strategy, can I class these data as paired?
I have performed chi-squared analysis, that states the differences are significant, but I am unsure how to interpret this as it does not tell me whether the implementation of the strategy was instrumental in the difference.
I would be happy to give any more information if needed and would really appreciate any thoughts/advice on my query.
Many thanks,
Bill Nolan BSc BVetMed MRCVS
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As you describe it you should not class the data as paired. The issue of not knowing if the effect is due to the intervention or changes in the population/hospital characteristics is typical in epidemiology and it should be adressed by adjusting for as many potential confounders as possible in a regression model if you only have two timepoints of measurement (before/after) if you can split your data into more time points before and after the strategy was implemented and if you can pinpoint when this happened an interrupted time series analysis could be worth looking into as it is relatively common in studies looking at effect of interventions in health care systems.
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This is not my field. Jon Louis, my GA, would be interested but you would have to discuss with him. I'm happy to be the conduit if it will benefit him in his professional work.
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John - I'm not certain as to what it is you're asking. Can you please clarify. You can email me directly at twade@brocku.ca. Thank you.
Cheers,
Terry
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Hey lovely community,
can anybody help me out here? A friend of mine is searching for some sources where the main methods for the determination of bacterial count in the field of cosmetics and food analysis are explained. Couldn´t find so far what she was searching for. Would be very happy for any help. 
Many thanks!
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Hello Ruben Scheuring
These two articles regarding bacterial count methods in cosmetics and food analysis could be helpful.
Does anyone have Indonesian version of Pemberton Happiness Index or other happiness scale?
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I'm currently doing a research on happiness and found an article about Pemberton Happiness Index. It is said there that this scale has a good validity and reliability, I wonder if anyone has ever used the scale in Bahasa Indonesia. Thank you.
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Thank you Karen. Did you also use the additional 10 items of experienced well being?
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We have collected few hundred specimens of Odonata from different localities (in different seasons) of northern part of Bangladesh. We are trying to identify them.  In this connection, we would like to make sure whether we are on the right track or not in relation to their identification. We have prepared texts based on their traditional morphological characters (including photographs) for 4 species of dragonfly and 1 species of damselfly. We shall be happy if anybody could help us to confirm these 5 species from the texts.  
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Hi if you can sent the photographs to me, maybe I can help 
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Deadline is enclosed in the picture attached
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will spread it among my network. 
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I would be very happy if anyone could help to have a detailed tutorials about PNN classification easily that makes me to understand all necessary equations and parameters easily?
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Karwan Great! I am glad the documents are good for you.
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These would be used in a survey study, perhaps as pre- and post-measures and should hopefully be sensitive to changes due to an intervention.
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For a multidimensional measure of social connectedness, there are not many choices. This one has yielded good data in some of my past survey research: Lee, R. M., & Robbins, S. B. (1995). Measuring belongingness: The Social Connectedness and the Social Assurance scales. Journal of Counseling Psychology, 42(2), 232.
You might also consider unidimensional measures from "big" psychological theories, including the 'positive relations with other subscale from this paper (Ryff, C. D. (1989). Happiness is everything, or is it? Explorations on the meaning of psychological well-being. Journal of personality and social psychology, 57(6), 1069.) and the "relatedness' subscale of a measure designed to assess Deci & Ryan's basic psychological needs, found in this paper (Gagne´ , M. (2003). The role of autonomy support and autonomy orientation in
prosocial behavior engagement. Motivation and Emotion, 27, 199–223.)
Cheers!
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I'm using Ryff's Psychological Well-Being Scale, as well as Waterman's Questionnaire for Eudaimonic Well Being (QEWB). Those seem to be the two major tools.
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Corey Keys distinguished between psychological and social elements of eudaimonia, making it a potentially comprehensive measure. He built on the work of Ryff. These two elements of eudaimonia, along with hedonia are measured by the Mental Health Continuum. It is also available in a short form. 
Keyes, C. L. M. (1998). Social well-being. Social Psychology Quarterly, 61, 121-140. DOI: 10.2307/2787065
Keyes, C. L. M. (2002). The mental health continuum: From languishing to flourishing in life. Journal of Health and Social Behavior, 43, 207-222. DOI: 10.2307/3090197
Keyes, C. L. M. (2009). Brief description of the Mental Health Continuum Short Form (MHC-SF). Available: http://www.sociology.emory.edu/ckeyes/.
Keyes, C. L. M., Wissing, M., Potgieter, J. P., Temane, M., Kruger, A., & van Rooy, S. (2008). Evaluation of the Mental Health Continuum Short Form (MHC-SF) in Setswana speaking South Africans. Clinical Psychology and Psychotherapy, 15, 181-192. DOI: 10.1002/cpp.572
Lamers, S. M. A., Glas, C. A.W., Westerhof, G. J., & Bohlmeijer, E. T. (2012). Longitudinal evaluation of the Mental Health Continuum-Short Form (MHC-SF). European Journal of Psychological Assessment, 28, 290-296. DOI: 10.1027/1015-579-a000109
Lamers, S. M. A., Westerhof, G. J., Bohlmeijer, E. T., ten Klooster, P. M, & Keyes, C. L. M. (2011). Evaluating the psychometric properties of the Mental Health Continuum-Short Form (MHC-SF). Journal of Clinical Psychology, 67, 99-110. DOI: 10.1002/jclp.20741
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I am working with the transformed bovine lymphocytes. These cells are in the suspension and I am planning to transfect these cells with the gene of my interest. Cell in suspension is often hard to transfect. Once I tried to transfect them with the  reagent (Lipofectamin LTX and plus) which I had used for Vero 2-2 cells (adherent cells). But that didn't work. So, I was wondering if someone has tried to do anything like this. If yes, then can you write me an answer down below? 
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Hi,
You can select suitable transfection reagent for pDNA, mRNA, siRNA delivery from RJH Biosciences, Canada - http://www.rjhbiosciences.com/.
They specialize in transfection of hard-to-transfect cells.
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I´m starting on a paper addressing happiness vs. loneliness relation within a city region? Does anyone know good references for this from the empirical happiness study? Or even better would be ones operating in an urban environment
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hi Mikko,
You have received a lot of valid answers to your question. Still, I´d like to mention an interesting author, a pioneer in research on loneliness and social support.  Only last Tuesday, Jenny de Jong Gierveld gave a lecture at the Leyden Academy on Ageing and Vitality, on crosscultural perspectives regarding loneliness. and social relations  Prof. Gierveld has developed a loneliness scale in her sociological work, which today is used in many countries (1), and she is involved in several large scale longitudinal studies on social networks, such as the Netherlands Kinship Panel Study and the Generations and Gender Surveys. She is an expert on demographic and social aspects of ageing, with particular reference to:
- well-being at later age and its relationship with social relations,
- the role of exchange of social support and its relationships with living conditions (rural or urban) and family arrangements (2), (3).
Some of her publications :
(1) Gierveld, Jenny De Jong, and Theo Van Tilburg. "A 6-item scale for overall, emotional, and social loneliness confirmatory tests on survey data." Research on Aging 28.5 (2006): 582-598.
(2) Scharf, Thomas, and Jenny de Jong Gierveld. "Loneliness in urban neighbourhoods: an Anglo-Dutch comparison." European Journal of Ageing 5.2 (2008): 103-115. 
(3) Gierveld, Jenny De Jong, and Marjolein van Broese Groenou. "Quality of Marriage and Social Loneliness in Later Life." Encyclopedia of Quality of Life and Well-Being Research (2014): 5309-5312.
I hope this may help you, and I wish you the best..
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I am shopping around for cheap but yet efficient transfection agents for primary cortical and hippocampal culture neurons. I have been using Lonza's nucleofactor and the efficiency is high (40%). Downside, need high number of cells per transfection, expensive and electroporate before plating. With all the vendors telling me how great their new product is, anybody can recommend any other reagents that is worth my time or money? Try modified calcium phosphate (clontech) protocol before, was not too happy with the health nor the efficiency.
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The Mirus version of nucleofactor works pretty well. Very comparable to Nucleofactor with a little higher toxicity but difference is minor. Just plate more cells. Neuromag works but gets 2-3 cells per coverslip, similar to Lipofectamine.
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Hi, I am starting research on instruments to increase happiness on community and country levels, could you give me suggestions on related readings?
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Dear Lyudmyla,
The January 2015 issue of Perspectives in Public Health contains several relevant papers. I attach my guest Editorial for that issue, which covers about 30 years experience in one page. In 2013 I led the team at the UK's Academy of Social Sciences which produced for Parliament a short booklet of case studies around Mental Wellbeing that covers a range of settings and ages: I attach that too. Happiness varies with age (the work of my old colleague Michael Argyle), and probably also with national culture (that is the work of Daniel Kahneman in the USA).
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Thanks.
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Hello Joshua,
We've successfully used funny movies in our lab (http://miplab.epfl.ch/pub/eryilmaz1101.pdf  and http://www.ncbi.nlm.nih.gov/pubmed/24603023). In terms of pictures, there exists an updated version of the good old IAPS which has been validated in a number of ways. Recently they even added erotic stimuli (I saw a poster, not sure it's already out but you can email the people), if you wanted that type of positive emotion. It's called Necki Affective Picture Set http://naps.nencki.gov.pl/Site/Home.html
On top of that, there are the Geneva affective pictures which offerts various types of appetitive stimuli:
Music is also a good elicitor, you can ask Wiebke Trost for the stimuli (http://cercor.oxfordjournals.org/content/early/2011/12/15/cercor.bhr353.full).
All depends on your design and what emotion exactly you want to elicit. I wouldn'd go for gifts unless you're studying the reward system or pleasure (giving people sweet food will surely activate the opioid receptors in the NAccumbens). Reward is one type of positive emotion.
Good luck!
Ewa
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What research/researchers should I read? I'm interested especially in education.
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I'm interested in understanding the connection between well being and happiness. Is the obsession of happiness in the social media a new form of consumerism - something you can buy if you engage in this course or follow these daily steps?
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Hi, Shirley:
Social Media, Happiness, Purchasing...is really wonderful---perhaps if we add 'Giving' to such an interesting combination!  Few years ago, I wrote (Nadeem, 2009) an article on a similar topic on what makes some of us happy or will make some of us happy as part of consumerism---"purchasing equals happiness equals giving'.Please take a look at it!
Moreover, as we continue to come under undeniable influence of social media in almost every aspect of our lives---we have to say that it is here to stay (again perhaps as part of consumerism)!  A recent research study (Robinson and Martin, 2008) highlighted that---we see that little attention in the quality-of-life literature has been paid to data on the daily activity patterns of happy and less happy people!
Using ratings-scale information from time-diary studies, the authors examined the hypothesis that people who describe themselves as happier engage in certain activities (in current times---social media---perhaps?) more than those who describe themselves as less happy.
Based on 34 years of data collected by the General Social Survey (GSS) on social activities and media usage, the authors found that people who are happy report being more active in most social activities, in religion and in newspaper reading. On the other hand, happier people report less time watching television, a relation that holds after control for education, marital status and other predictors of happiness (in current times---social media---perhaps?)!
  • Nadeem, M. (2009). Purchasing Equals Happiness Equals Giving: How do you plan to spend your weekend? The Journal of American Academy of Business, Cambridge, Vol. 15, No. 1, September 2009.
  • Robinson, J., & Martin, S. (2008). What Do Happy People Do?. Social Indicators Research, 89(3), 565-571.
Hope this helps!
Nadeem
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I coordinate a research on "How fairy tales influence happiness." I would need some suggestions of articles in which I can find similar studies on this topic. I am also interested in tests to measure happiness.
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Most of the measures will be of related constructs such as "subjective well-being" or "satisfaction with life." Ed Diener's Satisfaction with Life scale is good and very short. But I think you are looking for an immediate response to reading (or hearing) a story, right? For that, I would use a direct measure of current affect or mood state. These will usually include more than one type of emotion, but that's probably a good thing. (What if the fairy tale makes me scared or depressed?) The "Positive and Negative Affect Scale," (PANAS) is a good example; the PANAS-X is a nice extension of the original. You can download it here using the link below.
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I have always used Microsoft Excel to create my single-case design graphs. But I am not too happy with it. Does anyone know of a good alternative software tool? Especially for creating multiple baseline design graphs?
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There is a smartish R Package by your former colleague Jürgen Wilbert. The package is called "scan" and is specifically designed to analyze single-case data.
It also allows you to make nice plots in no time at all. Please find some simple examples attached and consider that i have a bad taste when it comes to colors... The plots can look really nice. :)
See his project page for installation instructions. If you encounter problems installing or using the package, i am happy to help! ;)
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I'm looking to study eudaimonic happiness in secondary school settings and think its a reasonable gap in research. If you know of any publications that get into this please let me know!
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We have done some studies on orientations to happiness of primary a, but never published it. I wasn't satisfied with the scale - I'm not sure it is appropriate for young people, especially the meaningful life items (which could be considered as eudaimonia). I an not aware of any other studies with children.
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Hello Researchers, I need public a database on Arousal-Valence which is only based on Facial Expressions (e.g., Surprise, Sadness,...., Happiness) for my ongoing research on Emotion Profiling. Thanks. 
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I am not sure. May be NVIE database can be helpful in this regard.
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There's data showing that in the USA, the happiness gradient increases as people live further out - in the rural areas (Okulicz-Kozaryn & Berry, 2011). Having said this, the happiness of Americans is also known to plateau at a certain wealth level, and it seems that there's some sort of correlation between urban wealth and happiness - to a point, which then reverses, seeing people off into the periphery again (Montgomery 2013). This may explain why the US situation is contradicted in much of Europe and Asia. But what drives this? Is there a way we can locate this magical threshold? And what does it have to do with the city, with suburbia and rural and remote areas? Is it the quality of the environments and what they offer inhabitants? 
It's also interesting that the charter of New Urbanism appears to tackle this issue by making cities better for inhabitants. Does this raise the threshold? (CNU., 2001)
There are also neural correlates that are being discovered that fascinate me - especially in Europe, where this phenomena is being taken seriously. Really interesting correlates, that have implications for mental health (Pedersen, Mortensen & Preben, 2001;Haddad, Schafer, et al., 2014). Oft times this data is only taken from an isolated city or rural area, and therefore may be tainted by uncontrolled variables, like the quality of social housing or by the dominance of a single industry.
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MIKKO WECKROTH (mikko.weckroth@helsinki.fi) has studied the problem: SUBJECTIVE WELLBEING AND HAPPINESS INSIDE A CITY REGION – URBAN VILLAGES AND GATED COMMUNITIES.  I quote:
"Aim of the study was to acquire knowledge about personal values and preconditions happiness is based on. Furthermore, moving into certain community and the desired characteristics of a neighbourhood contributing happiness, were defined to represent these values as well Instrumentalization of the concepts “happiness” and “subjective wellbeing” was constructed as a synthesis composing from Erik Allardts "loving", "having" and "being", Ed Dieners subjective wellbeing SWB and Aristotelian virtue of ethics. These components were transformed into an internet based questionnaire which was delivered to the residents of the neighbourhoods to be studied. As a research framework for comparative case study, juxtapositioning was constructed between a secluded post-suburban “gated community” located among the orbital roads of the metropolitan area, and more heterogeneous “urban village” better attached to existing urban structure. Results indicate that there can be found some differences concerning both self-reported levels and perceived preconditions for happiness effecting in neighborhoods studied. All of this variance cannot be explained through socioeconomical variables. Results thereby suggest that cultural orientation can be seen as determinant for evaluating the components of happiness as well as locating choices."
This is done in Finland.
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BBC report says scientists have developed a mathematical equation that can predict momentary delight. They found that participants were happiest when they performed better than expected during a risk-reward task.
"We can look at past decisions and outcomes and predict exactly how happy you will say you are at any point in time," said lead author Dr Robb Rutledge from University College London.
What is your opinion about this research? Now that we have an equation which can we use it in survey research to replace a Likert scale for such questions? Any further comments will help. Thanks.
Robb B. Rutledge, Nikolina Skandali, Peter Dayan, and Raymond J. Dolan - A computational and neural model of momentary subjective well-being, August 4, 2014, doi:10.1073/pnas.1407535111
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dear Behrouz,
(Happiness = Reality/Expectations). It is a fact although as you said it is not a precise equation.
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Sometimes it's too complicated to discuss/analyse happiness because there is a huge range of definitions - from a huge range of knowledge fields and traditions - that very rarely can get connected or even compared. I would like some indications of classic/seminal papers/research that deal with happiness as a concept and try to connect it to consumption levels and lifestyles, for instance.
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Did you check the discussions on Bhutans gross national happiness? Maybe the research on quality of life or the discussions on general alternatives to development (and consumption), such as the "Good Life" (Buen Vivir, Vivir Bien) in Ecuador and Bolivia can contribute something, too.
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I am considering the link between cognition and emotion but more specifically how people's positive thinking (e.g. I can do this... or I can achieve this) actually impacts the outcome. Schools of philosophical thought are also welcome. I have read a lot with regards to Positive Psychology (Broaden and Build theory), folk psychology and self help literature, Wiseman, Neuroplasticity and Meditation studies but would like to see if I have missed any specific studies or research areas.
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Having self-confidence and other positive or self-empowering attitudes can obviously contribute to successful achievement in any area of endeavor. However, as long as we reject, deny, cover over, or distance ourselves from the conscious experience of negative, uncomfortable, or painful feelings, the energy in those rejected feelings remains divided from our positive self-definitions, and remains trapped and blocked in the subconscious, producing wasteful loss of potentially productive energy in various kinds of inner self-conflict, self-control, and self-numbing or desensitization to our actual feelings and experiential states. The only effective way to liberate ourselves from our debilitating anxieties and other negative, self-defeating emotions is to openly explore into their essential foundational message or fearful message, so that that inner resistance can be deeply understood, compassionately embraced, and, thereby, healed, transcended, or psychologically outgrown. To be optimally successful, creative, flexible, and spontaneous, in our functioning, we need to retrieve the energy blocked in our uncomfortable, painful, or "negative" feelings and experiential states by welcoming them back into the natural undivided wholeness of our conscious energy, as a healing process of self-unification, self-integration, nonjudgmental self-acceptance, self-compassion, which is true self-love. That transformational/liberating energy of true self-love should not be confused with the antithetical attitude of egocentric self-infatuation or unrealistic, boastful, grandiosity, which is actually a disguised compensation for opposite feelings of emotional insecurity, fearful anxiety, self-judgment, self-rejection, self-abuse, lack of true and full self-acceptance. Creative insight and successful functioning are also enhanced when we are willing to relinquish separate narcissistic/egocentric self-awareness in deeply invested, warmhearted caring communion with other individuals, which enables us to tap into a connective relational creative energy, beyond the separate self-preoccupied ego. Being open to an essential core level of our own being as mystery, beyond all preconceived presumptive knowledge, also enables us to tap into a zone of limitless transformational possibilities, new discoveries, liberating breakthroughs. That Great Mystery zone of creativity is covered over and blocked if we overly rely on predetermined knowledge or make premature presumptive conclusions, because we are afraid of uncertainty, unpredictability, lack of resolution, lack of immediate closure, lack of control.
I have recently published two books that discuss the process of developing greater levels of productivity and creative insight, and how that can revitalize and transform one's individual life, personal relationships, and society as a whole. The titles of my books are:
1) "Psychological Healing Through Creative Self-Understanding and Self-Transformation." (ISBN: 978-1-62857-075-5)
2) "Deepening Your Personal Relationships: Developing Emotional Intimacy and Good Communication." (ISBN: 978-1-61897-590-4)
These books are available from Amazon, Barnes and Noble, and my author/ publisher website, http://sbprabooks.com/MaxHammer I have also posted my inspirational/transformational blogs and links to my radio interviews on my author webpage, as well as provided a more detailed description of the books, myself, and my fellow authors.
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Is there any theoretical explanation?
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Hi Antonio,
You might have a look at the paper called "Does child care involvement increase or decrease distress among dual-earner couples?" on the bottom of page 3 of my profile. Bob
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I´m interested in studying happiness at organizations, but the problem is that I don´t know an organizational database that can provide me with data about happiness.
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Interesting topic. Why don't you start a research yourself and generate your own data? It would be a great research project. Good luck with it!
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Although imperfect, would endorphin measurements or fMRI of reward centers be an acceptable approach? Any other possibilities
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I think it might be helpful to start by asking whether you're more interested in hedonic or eudaimonic well-being. Hedonic well-being refers to the basic pleasure seeking-pain avoidance sort of well-being. In contrast, eudaimonic well-being refers to a more wholistic congruence of a self in which competence, autonomy, and relatedness are satisfied. This paper gives an excellent distinction between the two: http://www.annualreviews.org/doi/pdf/10.1146/annurev.psych.52.1.141
For hedonic well-being, it may be difficult to assess this sort of processing without reference to a particular stimulus. The measure that might tap a trait sort of approach motivation is the resting frontal EEG asymmetry. Greater alpha power in the right than left hemisphere implies greater activity in the left than right hemisphere, which is associated with approach processing. The reverse is more consistent with greater withdrawal processing as a whole. This measure can be also be taken as a response to various stimuli, but it's still common just to record a couple of minutes of EEG data over the F3 and F4 sites with eyes open and eyes closed to get a sense of a person's baseline asymmetry.
With respect to hedonic response to various stimuli, there are additional complications. Kent Berridge has described a tripartite model of hedonic activity comprising wanting, liking, and reward learning. Grossly, the first describes the drive that causes an organism to approach reward, the second refers to hedonic enjoyment of the stimulus itself, and the third denotes an organism's ability to learn contingencies leading to reward. Here is a summary of his work: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756052/
Many of the "reward center" fMRI work involving dopaminergic systems is better at assessing wanting than liking. Brian Knutson's Monetary Incentive Delay task is good at this: ftp://130.113.218.160/dalywilson/Papers/futurediscounting/Knutsen.pdf Behaviorally, David Zald's group has been developing the Expenditure of Effort for Rewards Task (EEfRT) as a measure of wanting, and the initial paper describing this task is here: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006598
There are a couple of psychophysiological measures that work reasonably well for assessing liking. My favorite is the postauricular reflex, a measure I've been researching over the past decade as an index of positive emotional processing; this works for both emotional pictures and sounds: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962877/ The zygomatic EMG is also a measure of limited utility for assessing reactivity to pleasant stimuli; however, it's fairly specific to stimulus content and tends to work only for women: http://shootingcupoche.com/publication/10600215_Emotion_and_motivation_II_sex_differences_in_picture_processing/file/d912f50b5bcda99856.pdf
I'm not familiar with good physiological or behavioral measures of eudaimonic well-being. However, such a construct may be relatively difficult to measure in the laboratory, given that it's not necessarily something that occurs in response to specific stimuli. I hope this helps you in your work on subjective well-being and happiness!
Are academicians/professors/researchers happy compared to people in other professions?
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http://unsdsn.org/files/2013/09/WorldHappinessReport2013_online.pdf Walk down the corridor 1000 times at random hours. Record the number of times other profs/researchers look you into the eyes, smile, and say hello.
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As a new academic, any time you pass me in the corridor I am smiling - for the first time in my working life I am paid to read and to think and to write. I recall a professor telling me that the best thing about being an academic is that you get to "write your own letter and post it" - rather than only reading other people's letters.
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Is happiness based on our individual choices, and the way we think about things, or on inherited predispositions?
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Another interesting way of conceptualising and measuring a key element of eudaimonic (psychological wellbeing) is Steger's concepts of the search for meaning and Meaning in Life-Presence. I consider presence of meaning in life, which conceptually includes purpose in life, to be a vital aspect of psychological wellbeing. Keyes et al. (2002), for example, in both exploratory and confirmatory factor analyses supported a higher order well-being factor with two distinct oblique factors: psychological and subjective well-being. The highest loading for subjective well-being (SWB) was positive affect and the highest for psychological well-being (PWB) was purpose in life.
Overall though, I'd describe eudiamonism as an aspect of wellbeing, rather than of happiness, which I consider a more emotionally loaded aspect better represented by measures of positive affect. I know others disagree and use the term happiness more broadly.
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I believe visual communication can compensate for the shortcomings in verbal communication.
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As the first and foremost element in design, I suggest witticism and playfulness.
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Dear
Could anyone please let me know the procedure of making fine resolution DEM from CARTOSAT image. I would be happy even if you can help me to send relevant literature to prepare DEM. 
My objective is to check the impact of LU/LC change on runoff.
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Vikram, you can follow the attached discussion page. It directly answers your question.
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Does anyone know about the model of functioning happiness. I do have the model but the history of the model itself is not clear. Can anyone help me?
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There have been a lot published.  I wrote a paper on clarifying all the different forms and models.  Ill attach it to this email for you! 
I contrasted all the different forms of happiness (the 5 times) and constructed a conceptual model for it at the end
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Hi,
I am asking about positive control for T cells which elevate the production of IL-10 and anti-inflammatory properties
I would be happy for reference
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Thank you Michael
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I am looking for a precise calculation for Bhutan Happiness Index (BHI). Now, I need to use the principle of Bhutan Happiness Index to calculate for my research Social Adaptation Index. Please clarify whether I can use Social Adaptation index to substitute for BHI?
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I don't know exactly what you are looking at to construct your social adaptation index but check the site below
and try to compare and contrast those with what you are constructing so as to observe the plausibility. Good luck!
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You have mention about experiments. I will be happy to know more about it.
thanks 
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Dear Mofizur.
In you question you refer to the methods of attribution. I thinking that you are referring to attribution theory. As you certainly know attribution theory deals with how we attributes causes (internal vs. external; stable vs. unstable; controllable vs. uncontrollable to our and others performances. The theory was formulated mainly by B. Weiner. I wonder if attribution theory can be transferred to physical events, such as extreme weather  events in Bangladesh. In what follows, I elaborate a bit on attribution theory and hope that you can apply it to your research interests.
 As you know attribution theory has become a dominant paradigm, namely, in social psychology. Psychological research into attribution began with the work of Fritz Heider in the early part of the 20th century, subsequently developed by others such as Harold Kelley and Bernard Weiner. Three dimensions play a central role in this theory.
The first is consensus information, or information on how other people in the same situation and with the same stimulus behave. The second is distinctive information, or how the individual responds to different stimuli. The third is consistency information, or how frequent the individual's behavior can be observed with similar stimulus but varied situations. From these three sources of information observers make attribution decisions on the individual's behavior as either internal or external. There have been claims that people under-utilise consensus information, although there has been some dispute over this issue.
There are several levels in the covariation model: high and low. Each of these levels influences the three covariation model criteria. High consensus is when many people can agree on an event or area of interest. Low consensus is when very few people can agree. High distinctiveness is when the event or area of interest is very unusual, whereas low distinctness is when the event or area of interest is fairly common. High consistency is when the event or area of interest continues for a length of time and low consistency is when the event or area of interest goes away quickly.
Weiner's achievement attribution has three categories:
stability (stable and unstable)
locus of control (internal and external)
controllability (controllable or uncontrollable)
In attribution theory we often find a reference to the fundamental attribution error. The fundamental attribution error describes the tendency to overvalue dispositional or personality-based explanations for behavior while under-valuing situational explanations. The fundamental attribution error is most visible when people explain and assume the behavior of others. For example, if a person is overweight, a person's first assumption might be that they have a problem with overeating or are lazy and not that they might have a medical reason for being heavier set.
The core process assumptions of attitude construction models are mainstays of social cognition research and are not controversial—as long as we talk about "judgment". Once the particular judgment made can be thought of as a person's "attitude", however, construal assumptions elicit discomfort, presumably because they dispense with the intuitively appealing attitude concept
A self-serving bias is any cognitive or perceptual process that is distorted by the need to maintain and enhance self-esteem, or the tendency to perceive oneself in an overly favorable manner. It is the belief that individuals tend to ascribe success to their own abilities and efforts, but ascribe failure to external factors. When individuals reject the validity of negative feedback, focus on their strengths and achievements but overlook their faults and failures, or take more responsibility for their group's work than they give to other members, they are protecting the ego from threat and injury.
These cognitive and perceptual tendencies perpetuate illusions and error, but they also serve the self's need for esteem. For example, a student who attributes earning a good grade on an exam to their own intelligence and preparation but attributes earning a poor grade to the teacher's poor teaching ability or unfair test questions is exhibiting the self-serving bias. Studies have shown that similar attributions are made in various situations, such as the workplace, interpersonal relationships, sports, and consumer decisions.
Both motivational processes (i.e. self-enhancement, self-presentation) and cognitive processes (i.e. locus of control, self-esteem) influence the self-serving bias. There are both cross-cultural (i.e. individualistic and collectivistic culture differences) and special clinical population (i.e. depression) considerations within the bias. Much of the research on the self-serving bias has used participant self-reports of attribution based on experimental manipulation of task outcomes or in naturalistic situations. Some more modern research, however, has shifted focus to physiological manipulations, such as emotional inducement.
In what follows I elaborate a bit more on the three dimensions of attribution theory: consensus, distinctiveness and consistency.
Consensus refers to the extent to which other people behave in the same way in a similar situation (e.g., Mary smokes a cigarette when she goes out for a meal with her friend.  If her friend smokes, her behavior is high in consensus. If only Mary smokes it is low.
Distinctiveness has to do with the extent to which the person behaves in the same way in similar situations.  If Mary only smokes when she is out with friends, her behavior is high in distinctiveness. If she smokes at any time or place, distinctiveness is low.
Consistency refers to the extent to which the person behaves like this every time the situation occurs.  If Mary only smokes when she is out with friends, consistency is high. If she only smokes on one special occasion, consistency is low.
 Best regards, Orlando
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Hey everyone
I am using the ddRADSeq method for my research question. I build my libraries (double indexed - Illumina index and inline barcode) and ran it on the NextSeq as test run. Now I want to load the library again to get more reads per sample.
The problem is that the bioinformatic analysis of my test run results in very less loci shared among just a few samples. So, I think my range of the size selection was to large. Can I run my libraries on a gel and repeat the size selection step? Does is cut the libraries when I use this method of size selection? Would you prefer a Pippin Prep?
Has anyone done that before with success?
I would be great if someone has an answer to that.
Thanks a lot and Happy Easter.
Julia
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Hey Julia!!
I have done size selection after both library building and library pooling and both worked fine. But I used Pippin Prep - setting a tighter range. My reads on target increased a lot :) With gel, I don't know - but should work anyway!
Hope you are doing great!
Hugs,
R
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Hi to all dear researchers,
 and Happy New Nourooz
I am working on Green Cities, and need a map which shows the amount of CO2 existence in Iran atmosphere.
I need to find out which regions have more green gases and which areas contain less. 
Many Thanks for you time
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Dear James Varghese,
Many Thanks for your fast fine response.
Best Wishes
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I need to get English transcriptions of short (~5-7minute) interviews that were recorded in Spanish.  There are many services online, but I don't know how to determine which are more trustworthy or legitimate.  I want one that is reputable, and which I can trust with my data.  I've used Rev.com for English-English transcriptions, and I've been very happy with them, but they don't do Spanish-English transcriptions (only written translations), so they are not an option for my research needs.
Any help would be appreciated!
Thanks,
Rebecca
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You can use Youtube! for Spanish to English Audio lessons
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In the context of Bangladesh and nondemocratic countries, what is the matter for development? I would be happy to receive your constructive comments.
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Mr. Mollah,
Any polytical system is matter for development. Or the economic development is weakly affected by the type of polytical system.
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Greetings. I'm currently trying to culture B16F1 cells. But I observed a black spot/shadow in the culture flask. Hereby I attached two pictures from different culture flask, different batch and everything. There's no change of color in media. The black spots are not moving. I'm wondering if it is some kind of contamination, or cells overlapping, yeast or debris? Or is this practically normal for B16F1 cell line as I read some cell line got different shape? I'm the first student working with this cell line in my group. Unfortunately I'm not very familiar with cell works. I would be very happy if you could identify that black thing in my flask. Thank you. 
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Not all contamination will change the medium color, the color only indicates the pH of the media. Your black dots don't look like a contamination to me, but your cells are over confluent so it's hard to tell.
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I have used videos and pictures recommended for this purpose, but they did not work. 
Thank you
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Hello Carmen,
You have four classic ways to trigger emotions:
- recall of an affectively salient event
- script-driven guided imagery
- viewing images (+ music) or video
- posing affective facial actions, body postures, and vocal expressions
Zhang et al. (2014) provide a comparison of theses methods but according my experience video elicitation is the best from far.
There is also a 5th method which is performing small emotional task as wizard of oz games (Tcherkassof et al., 2013) or to put the hand in a jar full of spaghetti (Sneddon et al., 2012).
good luck!
Ref: 
Zhang, X., Yu, H. W., & Barrett, L. F. (2014). How does this make you feel? A comparison of four affect induction procedures. Frontiers in psychology, 5, 689.
Tcherkassof, A., Dupré, D., Meillon, B., Mandran, N., Dubois, M., & Adam, J. M. (2013). DynEmo: A video database of natural facial expressions of emotions. The International Journal of Multimedia & Its Applications, 5(5), 61-80.
Sneddon, I., McRorie, M., McKeown, G., & Hanratty, J. (2012). The belfast induced natural emotion database. IEEE Transactions on Affective Computing, 3(1), 32-41.
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I am going to do a research for understanding the experience of happiness in different generations, and would appreciate any insight or tips as to how to go about to find any kind of answear(even though I am well aware of how highly subjective this is). Any thoughts on how to develop a measurement that can help me draw general lines?
Best regards
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Happiness is a notoriously difficult concept to define. Everyone “knows” what happiness means in particular contexts but it has no single accepted meaning that can ground attempts to measure it. Researchers designing survey questions must either specify a meaning of happiness  which inevitably will fail to correspond to many individuals’ own ideas of happiness, or leave the definition open, which will lead to survey responses according to any number of incomparable versions of happiness. Happiness is an inherently vague and subjective experience which resists being forced into a numerical scale. People who are actually dissatisfied with their life will tend to answer that they are quite happy. Both ego defense and social desirability would cause such distortions. This bias is seen to manifest in over report of happiness; most people claim to be happy and most perceive themselves as happier than average. Hedonic level of affect can be assessed in three ways:
1) by direct questioning,
2) by projective tests and
3)by ratings based on non-verbal behavior
 A last method to be mentioned in this context is the focused interview of which the 'depth interview' is a variant. Such interrogations tend to cover all three happiness variants. A lack of clear reports on the themes of inquiry and on rating procedures mostly makes it difficult to assess their validity.
You construct deficient questions on happiness .There are also many questions that do-
Comparison with others
Better than in the past
Preference for another life
Difference with other satisfactions
Difference with other qualities of life.
What is the future of human's happiness - living in a smart city or living in nature?
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Which way of development will bring more happiness and health to people in the future: - Living in the spirit of progress in a modern urban environment - like smart cities? - Or living in an ecological way, in nature, among a clean and natural environment, away from the urbanized, city environment?  (I would appreciate it if you up-vote the question if you like it, as well as the answers you like)
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Through your question two beautiful quotes challenge me. They are a response to those who want to recognize them: “Nature made similar men, life makes them different”. Confucius “In nature, everything is always a reason. If you understand why, you do not need experience”. Léonard De Vinci Have we become slaves of the city and its attractions?
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In the Andean world of peasant agriculture, wisdom is love, nurturance, symbiosis, conversation, reciprocity, and dance. That wisdom may be comprehended by the Quechua expression Sumaq Kawsay, commonly translated as "good living", although we prefer "fullness of life". The notion of Sumaq Kawsay has been lately the subject of academic debate in Latin America, particularly in the Andes, since it has been chosen as the leitmotiv by Ecuadorian and Bolivian Constitutions in the past two years.
The Ecuadorian Constitution in particular introduces, for the first time, the idea of nature as a subject entitled to rights, in the same way as human beings. The academic and social groups in which the term was first used, aim to propose a new development model based in Andean cultural roots, built on respect, equality among all, solidarity, harmony and equity. 
We have found a similar notion, "Gawad Kalinga" ("to give care" in Tagalog) in the Philippines. Are there any other ancestral principles that are similar in other cultures? f
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Hi Francisco, I think that there is a similar set of ancestral principles among Aboriginal people in Canada. They are called the 7 sacred teachings or the 7 Grandfather's teachings. They include: love, respect, courage, honesty, wisdom, humility and truth. An example of what you are talking about with respect to nature would be that human beings have to respect mother earth. Clearly, climate change would be considered as consequence of science, technology and development that have taken place in the absence of respect for mother earth.
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Literature review in "Psychometric measuring of happiness" is an assignment for my Positive Psychology unite with 2500 t0 3000. I need to evaluate happiness measurements however I do not know how to categories the measurements in happiness then evaluate them. In addition How can I find out theoretical foundation of the scales ? , I need some scales which measure happiness in PP field as well. ( New bee in research) Thanks for your help Parisa
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Hi Parisa,
I agree with Dr. Joy in that happiness in research today seems to be synonymous (the same meaning) as subjective well-being. This tends to be measured in three ways: Satisfaction with life ( how 'happy' am I with my life currently), positive affect (how many positive emotions do I feel) and negative affect (how many negative emotions do I feel). Recently, Su, Tay and Diener (2014) created a new measurement instrument called the Comprehensive Inventory of Thriving that also helps capture some measures of Psychological Well-being. I've attached the paper related to this inventory. 
Also, my apologies for definitions in parentheses. I don't mean to offend if I've explained something you already understand.
Good luck to you!
Sincerely, Jim Smith
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Hi, I am culturing monocyte derived macrophages from human blood. For my assay I need to stain MDMs with Cell tracker green. Recently I get toxicity- cells literary turn secondary necrotic while staining or just after staining when I add complete media (staining done in serum free media). This would indicate some osmolarity/ pH problem so I made all new reagents, new vial of CTG with other source of DMSO and I still get this toxicity. I should add that cells are perfectly happy before and were not treated with anything. Anybody encounter something similar or have an idea what can be a problem? I run out of ideas... thanks
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Dear Agnieszka, this may happens because for some cell types change of the new medium may cause quite strong stress. Can you stain cell in the own medium without any washing changes? I think in this case, if you cell is happy in this medioum, you will not see necrosis.
Best wishes!
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Imagine you need to induce (psychosocial) stress in an individual enclosed within a space sized 2x3 meters. How would you do that? For the purpose of our experiment, we need to compare precisely defined individuals within enclosed space and we need to develop a stressed and non-stressed cohort... will be happy for any suggestions, Julie
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Besides the JD-R, psychosocial stress is typically conceptualized as a "stressor to strain" process model. See Karasek's "Job Strain" models, Hobfoll (COR Theory), and the JD-R, with a link below. http://www.beanmanaged.com/doc/pdf/arnoldbakker/articles/articles_arnold_bakker_159.pdf
Best regards!
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Imagine you need to develop a stress model (possibly psychosocial stress) in an individual closed in a chamber of size of an average room. How would you induce psychosocial stress? We excluded timed physical activity (ergometry etc), as it inteferes with our calculations. We don't even know whether such model exist - we need to compare the "stressed" individuals with the "non-stressed" ones under precisely described conditions. Will be happy for any suggestions, Julie
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Hello Julie
When I read your question, I thought of spaceflight, although a lot of the work looks at the physiology related to lack of gravity etc. but does not mention models re the psychosocial aspect. I don't know if this paper is of any interest. It looks as if David Dinges is the only author on ResearchGate; I don't know if he would be able to help:
Basner, M., Dinges, D. F., Mollicone, D. J., Savelev, I., Ecker, A. J., Di Antonio, A., ... & Sutton, J. P. (2014). Psychological and behavioral changes during confinement in a 520-day simulated interplanetary mission to Mars. PloS one, 9(3), e93298.Basner, M., Dinges, D. F., Mollicone, D. J., Savelev, I., Ecker, A. J., Di Antonio, A., ... & Sutton, J. P. (2014). Psychological and behavioral changes during confinement in a 520-day simulated interplanetary mission to Mars. PloS one, 9(3), e93298.
Gabriel, G., van Baarsen, B., Ferlazzo, F., Kanas, N., Weiss, K., Schneider, S., & Whiteley, I. (2012). Future perspectives on space psychology: Recommendations on psychosocial and neurobehavioural aspects of human spaceflight. Acta Astronautica, 81(2), 587-599.
Three out of the four authors are RG members:
Arnaldi, K. M., Smith, G., & Thropp, J. E. (2015). Human Behavior During Spaceflight-Evidence From an Analog Environment. Journal of Aviation/Aerospace Education & Research, 25(1), 27.
I will be interested to read further responses.
Best wishes with the work you are doing,
Mary
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Hello,
I'm using dynamic stimuli (17 morphed pictures : neutral to emotional valence (happy,angry,etc)). In each trial, the morphing stimulus was presented for 500ms.
Currently, the onset of stimulus is on the first picture (neutral). Consequently I can't link ERP to the emotional processing.
My question is : Could I place my onset on seventh (with emotional valence) picture for exemple ?
Thank you for your answer.
William
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Thank you for your answer Paul,
In fact, I would like to measure N1 and P300 from emotional faces but with my dyna;ics stimuli, the onset of trial (time=0) is on neutral face, consequently N1 is on neutral stimuli.
I would like to place my onset for N1 during trial (250ms) to measure N1 relative to emotional face.
Why not happy by education? Warum macht Bildung nicht unbedingt glücklich?
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I can understand that income needn't correlate with being fortunate, but why doesn't education predominantly lead to more happiness? (e.g.: A study by the british University of Warwick used a questionnaire  that measured mental wellbeing (see attachment) of 17.000 participants. Results showed that higher educated participants didn't score higher than less educated participants.) Dass höheres Einkommen nicht unbedingt glücklicher macht, kann ich nachvollziehen. Aber warum macht höhere Bildung nicht unbedingt glücklicher? (Bsp.: Eine Studie der britischen Universität Warwick leiß ca. 17.000 Personen einen Fragebogen zum psychischen Wohlbefinden (siehe Anhang) ausfüllen. Die Ergebnisse zeigten, dass gebildetere Menschen nicht unbedingt höher im Wohlbefinden abschneiden als weniger gebildete.)
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It might be due to non-realization of goals/ aims of education. Higher education as considered 'economic yield' if not fulfilled and expectation not fulfilled leads to frustration and ill mental health. 
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I am developing a  business model on new synergies in the licensing sector. Focus industry is fashion and luxury. I am happy about new input. Many thanks!
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dear Marie
you can see rhis paper
with best wishes 
dr.salim
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Hi there - I'm running assays on freshly isolated rat cardiomyocytes and am running into some issues with incomplete lysing of the cells. Cells are happy and contracting at time of plating and after agonist addition, but these cells stay completely intact after the addition of the assay lysis buffer. Is this a common thing? Is there an additive that will assist in the cell lysis without affecting the assay performance? Any insight would be much appreciated!!
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You could try 1% SDS for lysis and dilute it 10 times in the assay
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Is that about the scale or contradiction of idea?
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Hello
This site briefly discusses questionnaires including the Oxford Happiness Questionnaire:
This is also a comparison of various questionnaires of wellbeing:
Taggart, F., & Stewart-Brown, S. A Review of Questionnaires Designed to Measure Mental Wellbeing.
The authors acknowledge that there are equal numbers of negative and positive items in the Oxford Scale, but happiness can be indicated by not agreeing with the negative items.
Very best wishes
Mary
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I know DDVP is a good fumigant but i would prefer natural compounds that are registered fumigants since my assay is with natural compounds.
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I want to suggest that the bioassay to be used to test for the toxicity of the natural product is a determinant. This is because a positive control is know for its role in causing a well known effect via the assessment of biomarkers. Also the type of organism to be used is important, as specific response may be elicited by the test compound on different organisms. therefore it is not compulsory that a natural product will be used as positive control in your study.
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Hello,
I am going to conduct an ESM study, in which I intend to ask participants how happy they feel at the moment several times per day during two or three weeks, and what they are doing, in order to link an activity to a mean level of happiness for instance. (not unlike what researchers are doing with DRM studies)
I want it to be as simple as possible for the participants, so my intention is to only ask one single question about their happiness at the moment (and a question about what they are doing). Searching through the litterature about happiness and subjective well being (SWB), I have found quite a lot of different measures, but most are complete surveys with multiple questions (which I want to avoid) or deal with overall life satisfaction ("overall, how happy are you with your life?").
So far, I haven't found a valid and reliable single question that would measure SWB. I am well aware that, since there are several dimensions to it, it is more rigorous to ask multiple questions, but once again, I really want it to be as simple as possible.
What question should I ask ? Something like : "How happy are you feeling right now?" ?
And the study will be conducted with French participants by the way, so if someone here knows of a french formulation that works well, that would be very helpful (as you can imagine, it is much harder to find established measures in French than in English).
Thank you.
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Ok I will check this paper out, thank you !
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Hey ho,
I have a really hard time with cloning right now and happy for any advise.
I am using E. coli NEB5alpha and pET28a vector. The vector is PCR-amplified plus DpnI digested or digested with restriction enzymes NheI and XhoI. Both constructs results in a nice band on a gel and no colonies for my negative control.
For ligation, I am using the Gibson assembly for 15 min at 50°C. My insert is about 3000bp long, while my negative control is only water plus vector.
So as I said, there are no colonies for my negative control and >30 colonies for my insert, but my colony PCR results in only a band of about 300 bp (for the multiple cloning site) but not for the size of my insert. I have screened about 15 colonies now.
My PCR protocol accoding to NEB:
12.5 µL 2x Quick load master mix
1µl template ( I mix a tiny bit of a colony in 50 µL)
0.5 µL 10µM T7 forward primer
0.5 µL 10µM T7 reverseprimer
10.5 µl water
95°C 5 minutes
30 cycles of:
95°C 30 sec
45°C 1 minute
68°C 4 minutes (1minute/kbp)
68°C 5 minutes final extension
I hope anyone has suggestions. I have changed all reagents for PCR and my insert and vector are purified and the concentraion is fine. Maybe I miss / do something (obvious) wrong.
Thanks in advance!
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Sorry, your question does not meet my erpiriences.
Thomas Höpner